Antimicrobial Susceptibility Pattern of Coconut
Oil Extract on Selected Bacterial and Fungi
Volume 1 - Issue 3
Effiong Edet Bassey1*, Gwana Dam Mohammed1 and Okaro Cynthia2
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- 1Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria
- 2Department of Animal Health and Production Technology, Mahoment Lawan College of Agriculture Maiduaguri, Nigeria
*Corresponding author:
Effiong Edet Bassey, Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria
Received: April 10, 2018; Published: April 30, 2018
DOI: 10.32474/IPDOAJ.2018.01.000115
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Abstract
The study is aimed at evaluating the phytochemical content of coconut oil and determines the susceptibility pattern of coconut
oil against bacteria and fungi. Coconut oil is a high value coconut product and some positive health benefits have currently been
attributed to coconut intake such as its antioxidant and anticancer properties. Coconut oil has also been shown to have antibacterial
and antifungal properties and can be used in the treatment of infectious diseases. The findings revealed that the coconut oil sample
contains phytochemicals such as alkaloid, glycosides, Terpenoids and considered to be responsible for the many benefits attributed
to coconut consumption. This susceptibility testing was conducted by agar diffusion method and the zones inhibition indicated the
antimicrobial properties of coconut oil. The assey of antibacterial activity of standard bacteria organisms showed that Staphyloccus
aureus had the highest susceptibility to coconut oil while Pseudomonas aeroginosa had the least. this was concluded from their
average zones of inhibition 14.55mm (32%) for Staphyloccocus Aureus, 12.1mm (27%) for Streptocccus pneumonia, 10.95 (24%)
for Escherichia coli and 7.7mm (17%) for Pseudomonas aeroginosa. Candida albicans had a higher susceptibility to coconut oil more
than aspergillus in antifungal testing. This was also concluded from their average zones of inhibition 18.5mm (55%) for Candida
albicans and 15.1mm (45%) for Aspergillus fumigatus. The utilization of coconut oil should be promoted as a functional food in
Nigeria and the use of coconut seed flesh in our diets should be encouraged for health supporting functions.
Keywords: Antimicrobial; Susceptibility; Coconut Oil; Bacteria; Fungi
Abbrevations: NAFDAC: National Agency for Food and Drug Administration and Control; NA: Nutrient Agar; SDA: Sabaurauds
dextrose agar; MA: MacConkey agar; NB: Nutrient broth; SDB: Sabaurauds dextrose broth; MB: MacConkey broth; AST:Antimicrobial
Susceptiblity Testing
Abstract|
Introduction|
Materials and Methods|
Controls|
Phytochemical Analysis|
Antimicrobial Susceptibility Testing (AST)|
Results|
Discussion|
References|