Standardization of Synchronization Procedure to Collect the Similar Aged C. elegans

Volume 4 - Issue 1

Sarita Mishra, Aaditya Vikram Gaur and Rakhi Agarwal*

Received: February 28, 2020;   Published: March 05, 2020

DOI: 10.32474/PRJFGS.2020.04.000178

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Abstract

The selection of synchronized stage (same age group) is needed in any model organism viz worm, fly, zebrafish, those are having different developmental stages such as embryonic, larval, pupal then adult. Thus, synchronization is the key for the success of any toxicological experiments. C. elegans has been popularly used for the assessment of toxicity associated with pesticides and several other environmental toxicants. C. eleganslife cycle consists of embryonic stage, four larval stages and then adult stage. Thus, obtaining the similar age group worms will be helpful for different developmental, behavioural, and neuronal toxicity studies. Though, several synchronization protocols are already available however, they are complicated, time consuming, results in lesser yield and with more animal debris but in the present study we have shown a simplified, detailed protocol to collect the synchronized worms using swinging block rotors for centrifugation purpose.

Keywords: Synchronization C; Elegans; Bleach Treatment; Gravid Adult; Centrifugation with Swinging Block Rotors

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