Effect of Explant Type and Plant Growth Regulators Upon Callus Induction and Indirect Regeneration of Chickpea

Volume 4 - Issue 1

Hamzeh Minaei Chenar, Danial Kahrizi* and Alireza Zebarjadia*

Received: November 26, 2019;   Published: December 12, 2019

DOI: 10.32474/PRJFGS.2019.04.000177

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Abstract

Today protein shortage is one of the prevalent problems in the developing countries people’s food program specially the groups with low income. But chickpea production is limited because of biotic and abiotic stresses. Traditional breeding methods for some reasons are time consuming and are not very efficient. It is necessary to improve quality and quantity of chickpea via genetic engineering and gene transfer. Chickpea regeneration is prerequisite for gene transformation. The chickpea indirect regeneration like other legumes is limited. In this study callus induction and indirect regeneration was investigated in Bivanij cultivar of chickpea that is one of the best chickpea cultivars. For this purpose, four explants including embryo, cotyledon, node and hypocotyl were used. These explants were cultured on the MS medium with the best growth regulators compositions that have been previously reported. Also, the inducted calli from each explant were cultured on special regeneration medium. At the stage of callus induction, for cotyledon, hypocotyl and node, MS medium supplemented with 0.5mg/l BAP+0.5mg/l NAA showed the highest response. The embryos response was well for all mediums. The obtained calli from cotyledon(29.36%,), embryo(37%) and node(29.66%), showed the highest response for indirect regeneration on MS medium supplemented with 2mg/l TDZ and hypocotyl calli showed the highest regeneration (16%) on MS medium supplemented with 2mg/L BAP+0.125mg/l IBA.

Abstract| Introduction| Material and Methods| Results| Discussion| References|