Antimicrobial agents are defined as those substances which possess inhibitory effects against gram-positive and gram-negative bacteria, preventing their growth. Antibacterial agents are classified based on the type of action, source of antibacterial agent, and range of the spectrum. Rose belongs to the family Rosaceae. Hundred (n=100) species of roses are locally available. Roses are native primarily to the temperate regions of the Northern Hemisphere. There is a wide variety of colors available in roses which enhances the beauty of the rose. Most roses are locally available in Asia, North America and few are available in Europe. Many parts of rose have been used to check the antibacterial and antifungal property and they are highly active to remove toxins from the body and shown positive results to inhibit the growth of bacteria. Different parts of rose can be used separately against bacteria. Rose extract can also be used to treat sore throat and to relieve chest congestion. The agents which are responsible for the antibacterial and antifungal properties of the rose extract are flavonoid and phenolic compounds. The rose petals are powdered and mixed in distilled water and concentrated using a rotary evaporator. Pure cultures were swabbed on MHA plates and wells were made using sterile borer by agar well diffusion method. Twenty (20ul) of the extract was added in well and incubated at 37°C for 24 hours. Next day zone of inhibition was observed. To compare the activity of rose extract with amikacin, pure cultures were swabbed on MHA and amikacin disk was placed on each plate. Next day zones were observed. Rose extract has shown maximum inhibitory effects against Staphylococcus aureus whereas the inhibitory effects against Bacillus cereus, Bacillus subtilis, and Klebsiella pneumoniaewere not satisfactory. We have also observed that Vibrio cholera also showed sensitivity to rose extract whereas E.coli and Pseudomonas aureginosagave intermediate zones, therefore we suggest that we can use rose extract against Staph aureus, E.coli, Pseudomonas aureginosa and Vibrio cholera.
Keywords: Rose extract; Antibacterial activity; Sensitivity; Resistance; Disc diffusion