Whole-Cell Assays for Discovering Novel Efflux Inhibitors
for Use as Antibiotic Adjuvants
Volume 1 - Issue 2
Ellie Chapman and Sanjib Bhakta*
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- Department of Biological Sciences, Institute of Structural and Molecular Biology, University of London, UK
*Corresponding author:
Department of Biological Sciences, Institute of Structural and Molecular Biology, University of London, UK
Received:August 30, 2019; Published: September 06, 2019
DOI: 10.32474/CTBM.2019.01.000109
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Abstract
Antimicrobial resistance (AMR) is a growing problem worldwide. Resistance to antibiotics can occur in a number of ways, one
of which is removal of the drugs from the cell via efflux pump macromolecular machineries. As such, efflux pumps can provide a
background level of resistance to many different classes of antimicrobials and are a major contributor to AMR. Inhibition of efflux
pumps therefore has the potential to reverse resistance to many antibiotics in one go and is an attractive potential for treating
resistant infections. Whilst a number of efflux inhibitors are known, none are currently used clinically due to harmful side effects.
Development of novel inhibitors is therefore imperative. The article aims to review accumulation assays and efflux assays, two of
the most common laboratory techniques used to identify and characterise candidate efflux inhibitors.
Keywords:Efflux pumps; Efflux inhibitors; Efflux assays; Antimicrobial resistance; Drug discovery
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