Evaluation of D-amino Acid in Combination with Flucloxacillin on the Formation and Dispersal of Staphylococcus aureus Biofilm

Volume 1 - Issue 5

Khalid Faisal Alahmadi¹, Abdulrahman Mohammed Alalyani¹, Salman Ali Abdali¹, Emad Alharbi¹, Abdulaziz Jafar almusallam¹, Khalid Ibrahim Alahmadi² and Ammar AL-Farga³*

Received: October 10, 2019;   Published: October 16, 2019

DOI: 10.26717/CTBB.MS.ID.000123

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Abstract

Staphylococcus aureus is one of the most common bacterial strainsand has been linked to various infections ranging from light respiratory and skin infections to fatal conditions such as endocarditis osteomyelitis and periodontitis. However, S. aureus has been resistant to antibacterial drugs and body immune system response mechanisms owing to its efficient adaptation mechanisms such as biofilm formations. D-Amino acids are believed to play a significant role in the structural formation of the biofilm’s peptidoglycan. The amino acids form the peptide chains and crosslinks with N-acetylmuramic acid. Altering the structural composition of the compounds would compromise the mechanical integrity of the biofilm and, thus, could be used as a potential target for preventing biofilm formation. The aim of the given study is to evaluate the effect of D-amino acid in combination with flucloxacillin on the formation and dispersal of Staphylococcus aureus biofilm. This is achieved by determining the minimum inhibitory and lethal concentrations of D-Amino acids (D-glutamate, D-aspartate and D-methionine) in combination with flucloxacillin against Staphylococcus aureus. The effectiveness of D-Amino acids and flucloxacillin is investigated separately and the results compared. D-aspartate and D-methionine show antibacterial activity whereas D-Glutamate promotes bacterium multiplication. The minimum lethal concentration of flucloxacillin is 1.25mg/mL, whereas the minimum inhibitory concentration was 0.625mg/mL. D-methionine improved the flucloxacillin’s antibacterial efficacy and demonstrated a potential clinical significance by ANOVA test.

Keywords: D-amino acid;Staphylococcus aureus;Flucloxacillin

Abstract| Introduction| Materials and Methods| Results| Discussion| Conclusion| References|