Intervarietal Hybridization and Genetic Diversity of Rice by Molecular Markers

Diploma Debbarma and VK Khanna*

Received: June 22, 2018;   Published: July 05, 2018

DOI: 10.32474/CIACR.2018.03.000167

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Abstract

Cross ability of various accessions of Oryza sativa L. on the basis of pollen development and seed set was studied along with genetic diversity by RAPD and SSR markers. The variety Kalanamak had maximum number of fertile pollen (82.25%) whereas the variety Narendra-359 had the least number (61.03%). The cross of CAU R-1 with Hansraj (83.67%) gave the highest germination % after 1 hour. The cross of CAU R-1 with Narendra-359 gave the maximum seed set (68.96%). The pollen tube growth at 1 hour after pollination had a positive correlation with seed set. In general, selfing showed more pollen germination and pollen tube growth as compared to inter-varietal crosses with respect to seed set. Fourteen RAPD and eight SSR primers were used to assess the genetic diversity of 17 rice varieties. A total of 78 RAPD and 16 SSR amp icons were generated. The value of Jacaard’s similarity coefficient for RAPDs ranged from 0.284 to 0.766 with an average value of 0.526 whereas for SSRs it ranged from 0.071 to 1 with an average value of 0.396. The UPGMA cluster analysis grouped the 17 rice varieties in four clusters in case of RAPDs and three clusters in case of SSRs. The results of principal component analysis were comparable to the cluster analysis. In the Principal Component Analysis (PCA), for RAPD the first three components explained 65.86% of the total variation, with 53.21% explained by the first component and 7.23% by the second component, whereas for SSR the first three components explained 68.98% of the total variation, with 41.27% explained by the first component and 17.71% by the second component. The correlation coefficient and the significance of the correlation of the matrices based on RAPD and SSR data tested by the Mantel test showed that non-significant correlation (r=0.45) existed between both matrices.

Keywords: Crossability; Indica rice; pollen tube; Fruit set; RAPD; SSR

Abstract| Introduction| Materials and Methods| Result and Discussion| Results and Discussion| RAPD Analysis| References|