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ISSN: 2637-4676

Current Investigations in Agriculture and Current Research

Research Article(ISSN: 2637-4676)

FT-NIR Method to Identified Sugar Content in Honey Volume 9 - Issue 3

Mustafa A Al-Shamali*, Barak Al-Azmi and Fatima A Ali

  • Petroleum Research Center, Kuwait Institute for Scientific Research, Kuwait

Received: April 12, 2021;   Published: April 23, 2021

Corresponding author: Mustafa A Al-Shamali, Petroleum Research Center, Kuwait Institute for Scientific Research, Kuwait

DOI: 10.32474/CIACR.2021.09.000315

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A Fourier transformer near-infrared (FT-NIR) method has been created for analyzing the number of sugars (glucose, fructose, and sucrose) in honey that needs less sample preparation and is much faster than the conventional HPLC or FT-IR(Mid) technique. For this new method, the sample weight is not a critical parameter anymore. The method requires just as much sample as is needed to fill the 8mm sample vial. The FT-NIR model for predicting sugar in honey was developed from 55 syrup samples that contained glucose, fructose, and sucrose mixtures in different percentages as well as 8 honey samples. The amount of sugar in the 8 honey samples was determined by HPLC. The quality of the prediction model was checked by cross-validation. For the set of reference samples (55 syrups and 8 kinds of honey) the model has a high data correlation constant (R2) and low Root Mean Square Error of Cross-Validation (RMSECV <1). With time, the model can be updated each time the sugar content in a new honey sample is independently (other than FT-NIR prediction) measured. Our studies on several honey samples with unknown sugar content have shown that FT-NIR is very suitable for screening of honey and the obtained results were within the limit of the international standard results for sugar in honey (fructose‚ȧ60%, glucose‚ȧ45% for blossom Honey). The FT-NIR prediction has a very high reproducibility since fewer human errors caused by sample preparation can happen.

Abstract| Introduction| Theoretical and background| Equipment| Result and Discussion| Conclusion| References|