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ISSN: 2637-4676

Current Investigations in Agriculture and Current Research

Short communication(ISSN: 2637-4676)

Agronomic and Economic Performance of Bread Wheat (Triticum aestivum L.) Varieties in Response to N-Fertilizer Rate at Lemu-Bilbilo District, Southeastern Ethiopia Volume 9 - Issue 5

Khin Kyawt Yin2,3, Kanogporn Khammona1, Arweewut Yongsuwan1, Naraporn Chaomueang1, Siwaret Arikit4,5, Samart Wanchana1, Jintana Unartgam2 and Vinitchan Ruanjaichon*1

  • 1National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
  • 2Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen campus, Nakhon Pathom 73140, Thailand
  • 3Thailand Graduate Institute of Science and Technology (TGIST), National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani, Thailand
  • 4Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • 5Rice Science Center, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand

Received: July 26, 2021;   Published: August 20, 2021

Corresponding author: Vinit chan Ruanjaichon, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phenytoin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand

DOI: 10.32474/CIACR.2021.09.000321

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Abstract

Sweetness is an economically important eating quality trait for sweet corn breeding. The various types of sweet corn are caused by single gene mutation [1]. The brittle2 and shrunken2 mutants accumulated 20% of starch found in the corn kernels [2, 3]. Due to mutation in ADP-glucose pyro phosphorylase reveals as a key regulatory enzyme to limit the rate of starch biosynthesis pathway [4-7]. The brittle2 (bt2) mutant is classified as class 1 super sweet corn [3,8,9], having 15-30% sugar of total carbohydrate content in the kernel, while normal corn contains about 3% sugar in the kernel [9,10]. Newly, the functional marker based on shrunken2 gene (sh2) was identified and developed [11]. This SNP marker designed within the coding region in exon 1 at position 154 of the coding sequence was clearly separated the sh2-based sweet corn from waxy corn, field corn, and the sweet corn that was based on the other genes, i.e., su1, se1, and bt2. However, there is no report of SNP markers associated with bt2 in sweet corn.

In this study, we scanned for SNPs from an Axiom® Maize 600k Genotyping Array from a collection of 6 sweet corn and 9 waxy corn recombination inbred lines (RILs). The SNPs were used to evaluate linkage-disequilibrium (LD) decays and association with bt2. Additionally, SNP-based makers tightly linked to bt2 were validated to find a marker associated with super sweet kernel trait that could aid in future super-sweet corn improvement programs.

Keywords: Maize; kernel Sweetness; Brittle 2; Single Nucleotide Polymorphism (SNP)

Background| Results| Funding| Data Availability Statement| Acknowledgments| Conflicts of Interest| Reference|

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