Nanotechnology in Phytotherapy: The Effect of Noni’s
Nanoemulsion on Bacterial Translocation Induced By
Experimental Model of Intestinal Ischemia and Reperfusion
Volume 1 - Issue 1
Giovanna Dantas Fulco1, Irami Araújo-Neto2, Maria Helena Wanderley Costa Dantas3, Tarciso Bruno
Montenegro Sampaio4, Amália Cinthia Meneses do Rêgo5 and Irami Araújo-Filho6*
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- 1Postgraduate Program in Biotechnology, Potiguar University, Brazil
- 2Undergraduate Student of the Medicine Course, Potiguar University, Brazil
- 3Undergraduate Student of Nutrition Course, Potiguar University, Brazil
- 4Adjunct Professor, Department of Biology, Potiguar University, Brazil
- 5Post Graduation Program in Biotechnology, Potiguar University, Brazil
- 6Full Professor of the Post Graduate Program in Biotechnology at Potiguar University - Laureate International Universities, Department of Surgery, Potiguar
Dr Irami Araújo Filho, Full Professor of the Post Graduate Program in Biotechnology at Potiguar University - Laureate International
Universities, Natal, Rio Grande do Norte, Full Professor, Department of Surgery, Federal University of Rio Grande do Norte. Full Professor, Department of
Surgery, Potiguar University, PhD in Health Science, Brazil.
Received: January 09, 2018; Published: January 19, 2018
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Background: The study evaluated nano structured extract Morinda citrifolia L. (Noni) conveyed in enteral form, in an experimental model
of intestinal ischemia/reperfusion (I/R), as well as in the prevention of bacterial translocation.
Purpose: Observe the protective and repairing effect of Noni’s nanoemulsified extract in the presence of induced bacterial translocation,
by an experimental model of intestinal ischemia/reperfusion.
Methods and Findings: The study consisted of 2 groups of 7 animals, where the Controls were treated with 0.9% saline solution (I/R +
S) and Experimental group (I/R + Noni) treated with 5 mg/ml/Kg Noni nanoemulsion, orally by gavage 12h and 2h before the experiment.
In the groups, the superior mesenteric artery was occluded with a vascular micro clamp and the laparotomy reopened 60min. after for pull
back the clamp. Treatment response was assessed by blood count, inflammatory markers and biochemical dosages, including a sample of the
terminal ileum and hepatic of each animal was harvested, fixed in formalin 10% and included in paraffin and stained with hematoxylin-eosin
for morphometric measurement. Measurement of TNF-α, IL-1β, IL-6, and IL-10 was done. One gram of spleen, liver, and mesenteric lymph
nodes were harvested for culture by selective means for Gram (-) and Gram (+) bacteria. ANOVA and the post-hoc Turkey and Student´s t test
were used, considering p<0, 05 as significant.
Conclusion: Noni´s nanoemulsion positively influenced the organic reactions in the presence of intestinal ischemia/reperfusion, reducing
the production of pro inflammatory cytokines, bacterial translocation, preventing tissue injury and attenuating the systemic inflammatory
response against the experimental model used
Keywords: Noni: Ischemia-reperfusion injury; Bacterial translocation; Biotechnology; Nanotechnology; phytotherapy
Abbreviations: GGT: Gamma-glutamyl Transferase; ECUA: Ethics Committee on the Use of Animals; NCCAE: National Council for Control of
Animal Experimentation; NO: Nitric oxide; AST: glutamic-oxalacetic transaminase; ALT: glutamic-pyruvic transaminase; HSV: total bilirubin and
fractions, erythrocyte sedimentation rate; SMA: Superior Mesenteric Artery
Material and Methods|