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ISSN: 2637-6679

Research and Reviews on Healthcare: Open Access Journal

Research Article(ISSN: 2637-6679)

Nanotechnology in Phytotherapy: The Effect of Noni’s Nanoemulsion on Bacterial Translocation Induced By Experimental Model of Intestinal Ischemia and Reperfusion

Volume 1 - Issue 1

Giovanna Dantas Fulco1, Irami Araújo-Neto2, Maria Helena Wanderley Costa Dantas3, Tarciso Bruno Montenegro Sampaio4, Amália Cinthia Meneses do Rêgo5 and Irami Araújo-Filho6*

  • Author Information Open or Close
    • 1Postgraduate Program in Biotechnology, Potiguar University, Brazil
    • 2Undergraduate Student of the Medicine Course, Potiguar University, Brazil
    • 3Undergraduate Student of Nutrition Course, Potiguar University, Brazil
    • 4Adjunct Professor, Department of Biology, Potiguar University, Brazil
    • 5Post Graduation Program in Biotechnology, Potiguar University, Brazil
    • 6Full Professor of the Post Graduate Program in Biotechnology at Potiguar University - Laureate International Universities, Department of Surgery, Potiguar University Brazil

    *Corresponding author: Dr Irami Araújo Filho, Full Professor of the Post Graduate Program in Biotechnology at Potiguar University - Laureate International Universities, Natal, Rio Grande do Norte, Full Professor, Department of Surgery, Federal University of Rio Grande do Norte. Full Professor, Department of Surgery, Potiguar University, PhD in Health Science, Brazil.

Received: January 09, 2018;   Published: January 19, 2018

DOI: 10.32474/RRHOAJ.2018.01.000101

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Background: The study evaluated nano structured extract Morinda citrifolia L. (Noni) conveyed in enteral form, in an experimental model of intestinal ischemia/reperfusion (I/R), as well as in the prevention of bacterial translocation.

Purpose: Observe the protective and repairing effect of Noni’s nanoemulsified extract in the presence of induced bacterial translocation, by an experimental model of intestinal ischemia/reperfusion.

Methods and Findings: The study consisted of 2 groups of 7 animals, where the Controls were treated with 0.9% saline solution (I/R + S) and Experimental group (I/R + Noni) treated with 5 mg/ml/Kg Noni nanoemulsion, orally by gavage 12h and 2h before the experiment. In the groups, the superior mesenteric artery was occluded with a vascular micro clamp and the laparotomy reopened 60min. after for pull back the clamp. Treatment response was assessed by blood count, inflammatory markers and biochemical dosages, including a sample of the terminal ileum and hepatic of each animal was harvested, fixed in formalin 10% and included in paraffin and stained with hematoxylin-eosin for morphometric measurement. Measurement of TNF-α, IL-1β, IL-6, and IL-10 was done. One gram of spleen, liver, and mesenteric lymph nodes were harvested for culture by selective means for Gram (-) and Gram (+) bacteria. ANOVA and the post-hoc Turkey and Student´s t test were used, considering p<0, 05 as significant.

Conclusion: Noni´s nanoemulsion positively influenced the organic reactions in the presence of intestinal ischemia/reperfusion, reducing the production of pro inflammatory cytokines, bacterial translocation, preventing tissue injury and attenuating the systemic inflammatory response against the experimental model used

Keywords: Noni: Ischemia-reperfusion injury; Bacterial translocation; Biotechnology; Nanotechnology; phytotherapy

Abbreviations: GGT: Gamma-glutamyl Transferase; ECUA: Ethics Committee on the Use of Animals; NCCAE: National Council for Control of Animal Experimentation; NO: Nitric oxide; AST: glutamic-oxalacetic transaminase; ALT: glutamic-pyruvic transaminase; HSV: total bilirubin and fractions, erythrocyte sedimentation rate; SMA: Superior Mesenteric Artery

Abstract| Introduction| Material and Methods| Results| Discussion| Conclusion| References|