Phytochemical Analysis of Euphorbia Hirta and Cytotoxic Activity on Ma104

Volume 2 - Issue 1

Ponnambalam Arun*, Kaveri Krishnasamic, Palani Gunasekeranc and Vidya Padmanabhan

Received: January 26, 2019   Published: February 01, 2019

DOI: 10.32474/PAPN.2019.02.000130

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Abstract

Diarrheal diseases continue to be largest and deadliest ones globally especially affecting children. From earlier days there is renewed interest in the discovery of novel compounds that can be used to fight these kinds of diseases using plants and its materials. Rotavirus has been recognized as the major etiological agent of diarrheal diseases of young children in developed as well as developing countries. The presence of various phytochemicals like flavonoids, tannins, saponins, alkaloids and terpenes present in the active extracts increase the colonic water and electrolyte reabsorption and result in treatment of diarrhea. This study was undertaken to know about traditional herbal plant having medicinal value. Euphorbia hirta was analyzed for the presence of phytochemical and cytotoxic activity using MA104 cell line. Non toxic dose of the extracts were used to determine their in-vitro inhibitory activity. Cytotoxic activity were assessed using (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT). The cytotoxicity from crude acetone extract of Euphorbia hirta had activity in non-toxic concentration. The potency of the plant extract concentration has been calculated using percent decrease in number of viable cells in MA104 compared with control value. This observation of potential activity on MA104 are worth for further studies and present study corroborates ethno pharmacological data as a valuable source in the selection of plants with antiviral activity and to some extent validates their traditional use.

Keywords:Phytochemical; Cytotoxicity; Euphorbia hirta; MA104; Antiviral activity

Abstract| Introduction| Euphorbia Hirta| Materials and Methods| In-vitro experiments| Results| Discussion| References|