Ethanol Neogenesis in Stored Blood

Volume 1 - Issue 3

Komal Singh¹*, Ritu Malik² and Loveleen Kumar Katyal²

Received: March 19, 2018;   Published: March 22, 2018

DOI: 10.32474/PRJFGS.2018.01.000111

Full Text PDF

To view the Full Article   Peer-reviewed Article PDF

Abstract

During the investigation of accidental cases, blood samples obtained from accidental victim are submitted to forensic science laboratory for toxicological analysis. All through, ethanol analysis is performed on almost all cases of blood samples. The main problem is determining alcohol concentration in these cases is changes in Blood Alcohol Concentration (BAC) that can occur due to neo formation of ethanol. Blood samples stored under in sufficient conditions or for longer times and body fluids have undergone petrifaction often contains certain amount of volatile compounds. In the light of possibility that, neo formation of ethanol in improperly stored blood might give rise to an incorrect estimation of BAC. With this in mind, to study the effect of preservative and temperature on the formation and concentration of ethanol in blood samples, we estimated the ethanol neo genesis in stored and normal blood in different time period (0, 7, 14, 21, 28, 35 and 42 days). Head Space-Gas Chromatography instrument used to measure the level of ethanol in blood sample. We found that neo formation of ethanol was observed in normal blood sample. There was no any neo formation observed in blood sample with preservative and blood stored at 4 °C. Our study is supporting the studies which suggest that storage condition will affect the neo formation of ethanol in blood samples.

Keywords: Blood Alcohol Concentration; Neo formation; Head Space-Gas Chromatography; Ethanol.

Abstract| Introduction| Drawback: This is also inhibitor of many enzymes and also effect urease for the estimation of urea| Aims and Objectives| Materials and Method| Results and Discussion| Conclusion| Acknowledgement| References|