Expression of Metalloproteinase-8 in Gingival Crevicular Fluid, Peri-Implant Sulcular Fluid and Saliva in Healthy and Diseased Periodontal and Peri-Implant Tissue. Volume 4 - Issue 5

Theodosia Lazaridou¹, Aikaterini Elisavet Doufexi*², Giorgos Menexes³ and Lazaros Tsalikis⁴

• ¹DDS, Graduate student, Department of Preventive Dentistry, Periodontology and Implant Biology, Aristotle University of Thessaloniki, Greece
• ²DDS, Ph D, Department of Preventive Dentistry, Periodontology and Implant Biology, Aristotle University of Thessaloniki, Greece
• ³PhD Associate Professor, School of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, Thessaloniki, Greece
• ⁴DDS, Ph D, Professor at the Department of Preventive Dentistry, Periodontology and Implant Biology, Aristotle University

Received: April 01, 2021   Published:April 29, 2021

Corresponding author: Aikaterini-Elisavet Doufexi, Department of Preventive Dentistry, Periodontology and Implant Biology, Aristotle University of Thessaloniki, Greece

DOI: 10.32474/MADOHC.2021.04.000197

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Abstract

The study aimed to compare the levels of Metalloproteinase- 8 (MMP-8) in saliva, Gingival Crevicular Fluid (GCF), and Peri Implant Sulcular Fluid (PISF) in health and disease before and after treatment of periodontal and peri implant diseases. 42 patients treated with dental implants were divided into 3 groups: 15 periodontally healthy patients with healthy implants (Group A), 14 patients with gingivitis and peri implant mucositis (Group B), and 13 patients with periodontitis and peri implantitis (Group C). Clinical recordings, sulcular fluid sampling, and saliva collection were performed before and 3 months after treatment. Immunological characteristics were compared using metalloproteinase-8 (MMP-8) protein levels results using Enzyme-Linked Immunosorbent Assay (ELISA). Therapy lowered the PPD, PPDmax, and PI values of teeth and PI values of implants only in group C. Levels of MMP- 8 in GCF and PISF were similar for all groups; MMP-8 levels in saliva of group B were statistically higher compared to the other groups. Saliva showed higher MMP-8 levels than GCF and PISF. In all groups and fluids, MMP-8 levels were statistically unchanged after treatment. The analysis of MMP-8 in saliva could potentially offer insight into the molecular pathways involved in periodontal and peri-implant tissue inflammation

Keywords: Collagenase; Oral fluids; Periodontal inflammation Peri-Implant inflammation Expression

Abstract| Introduction| Materials and Methods| Therapeutic Procedure| Results| The Effect of Treatment| Discussion| Acknowledgment| References|