“Phytochemical Screening, Anti-Oxidant and Anti- Hyperglycaemic Activity Determination of PHYLLANTHUS EMBLICA” Volume 3 - Issue 4

Leashini A/P Subramaniam, Nabila Perveen, and Naeem Hasan Khan*

Department of Pharmacy, AIMST University, Malaysia

Received: October 09, 2021   Published: November 05, 2021

*Corresponding author: Naeem Hasan Khan, Department of Pharmacy, AIMST University, 08100 Bedong, Kedah DA, Malaysia

DOI: 10.32474/OAJCAM.2021.03.000169

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Abstract

Present research was intended to study the phytochemical, antioxidant and antihyperglycemic activity of Phyllanthus emblica fruits ethanolic extract (PEFEE). This involved the preparation of PEFEE using cold maceration and soxhlet extraction, followed by its phytochemical analysis and antihyperglycemic and antioxidant activity. Maceration and soxhlet extraction of Phyllanthus emblica using ethanol offered dark brown of Phyllanthus emblica fruits ethanolic extract. The antihyperglycemic activity involved STZ induced diabetic rats where they were treated with PEFEE at 200 mg and 400 mg/kg body weight/day, or glibenclamide (10 mg/kg body weight/day) for 21 days. Phytochemical screening of the extract revealed the presence ophytoconstituents, such as: carbohydrates, proteins, amino acids, steroids, flavonoids, tannins and phenolic compounds, alkaloids, cardiac glycosides, coumarins, anthraquinone, flavonoids and volatile oils The antioxidant activity and total phenolic content wascarried out to identify the IC50 value and the total amount of phenolic content of Phyllanthus emblica. The DPPH radical scavenging assay and total phenolic test were shown positive for the antioxidant activity of the Phyllanthus emblica. As a basis, Folin-Ciocalteu reagent was used to measure the phenolic content of different concentrations of plant extract. In this test, the Gallic acid was used as a standard and the total phenolic content was calculated by using the standard formula In the present study, the total phenolic content found was to be 6.420 mg GAE/g and 6.097 mg GAE/g for the concentrations of 50 and 200μg/ml respectively. According to the results obtained, the IC50 was calculated for the BHT as well as Phyllanthus emblica extract, the value was 84.04μg/ml and 182.82μg/ml, respectively. However, it can be concluded that the Phyllanthus emblica are exhibiting antioxidant effect as it shows positive results for the total phenolic content (TPC) as well as DPPH scavenging method. The antihyperglycemic activity revealed that Phyllanthus emblica fruits extract has antihyperglycemic effect against STZ induced diabetic rats and the extract maintains the BW of diabetic rats. Present study established that the Phyllanthus emblica fruits ethanolic extract possess antihyperglycemic activity.

Keywords: Phytochemicals; antioxidant; Phyllanthus emblica; antihyperglycemic; Soxhlet

Abstract| Introduction| Materials and Methods| Results| Statistical Analysis| Discussion| Conclusion| Interests of conflict| Acknowledgement| References |