Berberine is an alkaloid class of drug which has enormous therapeutic potential, but the bioavailability is very poor (<1%) due to its low solubility and poor intestinal absorption. In this study, the influence of the Trivedi Effect® on the structural properties and the isotopic abundance ratio of berberine chloride was evaluated using LC-MS and GC-MS analytical techniques. Berberine chloride sample was divided into control and treated parts. Only the treated part was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mahendra Kumar Trivedi. The LC-MS spectra of both the samples observed at retention time 2.0 minutes and the molecular ion peak at m/z 336.25 [M]+ in the mass spectra. The peak area of the treated berberine was significantly increased by 4.42% compared to the control sample. The LC-MS based isotopic abundance ratio of PM+1/PMin the treated berberine was significantly increased by 34.4% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM in the treated berberine was very increased by 1547.15% compared with the control sample. Thus,13C, 2H, 15N, and17O contributions from (C20H18NO4)+ to m/z 337 in the treated sample were significantly increased compared with the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O) in the treated berberine was significantly altered compared to the control sample. The increased isotopic abundance might occur due to the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio of the Consciousness Energy Healing Treated berberine chloride may increase the chemical bond strength, stability, solubility, and bioavailability in the body. The new form of berberine chloride would be more efficacious novel pharmaceutical formulations against diarrhoea, gastroenteritis, bacterial and fungal infections, cancer, diabetes, arrhythmia, inflammation, hyperlipidemia, etc.