Isolation, Identification and Antibacterial Susceptibility of Microorganisms Collected from Live Birds Market in Sokoto Volume 4 - Issue 1

A A Ebbo* and Lawal Junaidu

• Department of Veterinary Pharmacology and Toxicology, Usmanu Danfodiyo University, Nigeria

Received:September 24, 2021;   Published: October 25, 2021

Corresponding author: A A Ebbo, Department of Veterinary Pharmacology and Toxicology, Usmanu Danfodiyo University, Sokoto, Nigeria

DOI: 10.32474/JCCM.2021.04.000176

 

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Abstract

Thickly populated Kerala was the first Indian state to get affected by COVID-19 on 2nd Feb 2020. The number of cases

Thickly Microbial resistance to most of the currently used antimicrobial agents is on the increase. This was studied in Sokoto by culture, isolation, identification and susceptibility testing of microorganisms from cloacal swabs in live birds. This was carried out in Veterinary Microbiology and Veterinary Pharmacology laboratories, Usmanu Danfodiyo University, Sokoto. The study was performed on 100 cloacal swab samples. The samples were collected from live birds and transferred aseptically to the laboratory. All the experimental work was done under sterile conditions. From the 100 cloacal swab samples, 97 isolates were gram negative and 3 were gram positive bacteria. Among all the isolates, 23 (23%) E. coli, 20 (20%) Salmonella spp., 22 (22%) Enterobacter spp., 16 (16%) Proteus spp., 4 (4%) Pseudomonas spp., 3 (3%) Shigella spp., Staphylococci spp., 3 (3%). In gram staining, both gram positive and grams negative bacteria were found. Isolation and identification were by observing colony morphology, grams staining and standard cultural and biochemical test. Diversified bacterial species were prevalent in cloacal swabs of broiler. However, E. coli and Salmonella spp., are of public health importance (zoonosis), so birds harboring these organisms become threat to human. It could be concluded that birds from live birds’ market in Sokoto harbor multi resistant organisms that may pass through the feces to the environment and cause potential human health hazards and can cause illness.

Keywords:Antimicrobial; Microbiology; Culture; Isolation; Identification; Susceptibility Testing

Abstract| Introduction| Materials And Methods| Isolation, Identification and Antibacterial Susceptibility| Results| Discussion, Conclusion, And Recommendations| References|