Caffeine Pretreatment Increases Survival of DBAXC57BL
Mice Exposed to Different Doses of γ- Radiation by
Glutathione Elevation
Volume 2 - Issue 1
Ganesh Chandra Jagetia1* and Manjeshwar Shrinath Baliga2
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- 1Maharana Pratap Colony, Hiran Magri, India
- 2Father Muller Research Centre, India
*Corresponding author:
Ganesh Chandra Jagetia10 Maharana Pratap Colony, Hiran Magri, India
Received: March 14, 2020; Published: June 19, 2020
DOI: 10.32474/CTBM.2020.02.000126
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Abstract
Humans are exposed to ionizing radiations from various sources including background, air or space travel and diagnostic and
cancer therapy. The deleterious changes induced by ionizing radiations can be reduced using different pharmacophores. The effect
of 80 mg/kg body weight of caffeine was studied on the radiation-induced sickness and mortality in DBAxC57BL mice exposed to 7
to 13 Gy of -irradiation. Treatment of mice with caffeine one hour before irradiation delayed the onset of mortality and reduced
the symptoms of radiation sickness when compared to saline treated irradiated controls. Caffeine provided protection against both
the gastrointestinal and hemopoietic deaths. However, animals of both the Caffeine and Saline pretreated irradiation groups did not
survive up to 30 days post-irradiation beyond 11 Gy irradiation. The LD50/30 was found to be 9.4 Gy for the Saline and 10.2 Gy for
caffeine pretreated irradiation group, respectively with a dose reduction factor of 1.1. The ability of caffeine to protect mice against
the radiation induced mortality is due to increase in glutathione accompanied by a reduced lipid peroxidation on 31days in the
survivors. Caffeine protected the DBAxC57BL mice against radiation induced sickness and mortality by increasing glutathione and
depleting lipid peroxidation.
Keywords: Caffeine; survival; dose reduction factor; glutathione; lipid peroxidation; radiation sickness
Abstract|
Introduction|
Materials and Methods|
BIOCHEMICAL ESTIMATION|
Analysis of data|
Results|
Discussion|
Conclusion|
Acknowledgements|
Conflict of interest statement|
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