Received:February 18, 2020;   Published: February 27, 2020

DOI: 10.32474/CIACR.2020.08.000286

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Abstract

In order to find out the effect of biogas slurry utilization in long-term on soil nutrient and soil enzyme activity, the typical breeding enterprises of biogas slurry utilization in Henan Province was investigated by April and October 2019. The results showed that the content of organic matter, nitrogen, phosphorus and potassium in biogas slurry was relatively low, including 0.26g/l of organic matter, 1.27g/l of total nitrogen, 0.19g/l of total phosphorus and 0.94g/l of total potassium. After years later by biogas slurry irrigation and application, the nutrient factors of the soil surface layer were improved, among which the organic matter in the soil surface layer increased by 0.2-27.9g/kg, the total nitrogen increased by 0.02-2.7g/kg, and the total phosphorus increased by 0.1-3.25g/kg the mode of combining the biogas residue with the biogas liquid of KANGLONG biogas was the largest one. Total potassium increased by 0.2-5.53g/kg, with an increase of 1.14-28.10% which of YOULIN was the largest one. The value of pH changed to conducive to soil nutrient release. The accumulation of available P in soil is increased by 9.18-108.7mg/kg, which is consistent with the trend of organic matter and total P content. The hydrolyzed N is increased by 0.2-156.79mg/kg, which is consistent with the trend of organic matter and total N content, and the available K is increased by 19.23-109.45mg/kg, which is consistent with the trend of total K. That all are the results of long-term accumulation by use of biogas slurry. The activities of soil hydrolase and oxidoreductase significantly were increased by use of biogas slurry. Compared with the control farmland, protease increased by 0.49-4.02IU/L, increased by 8.77-64.42%, invertase increased by 51.34-235.25IU/L, increased by 11.24-41.83%, urease increased by 71.78-374.54IU/L, increased by 10.55-49.12%, acid phosphatase increased by 1.49-14.77IU/L, increased by 5.16-45.86%, cellulase increased by 2.51-58.81IU/L, It increased by 2.72-61.40%, nitrate reductase by 0.48-9.82IU/L, 3.51-55.95%, nitrite reductase by 0.7312.89IU/L, 2.34-51.47%, dehydrogenase by 0.11-3.88IU/L, 3.51-62.19%, catalase by 2.93-32.77IU/L, 4.33- 46.18%. There was a significant correlation between soil nutrients and enzyme activity, among which soil organic matter, N, P, K, dehydrogenase and catalase activities were all positively correlated, soil total N, hydrolyzed N, available P and cellulase were also positively correlated, organic matter, available K and cellulase were significantly correlated. At the same time, there are significant or extremely significant correlation between different soil enzymes.

Keywords: Biogas slurry; Biogas slurry utilization; Soil nutrients; Soil enzyme activity; Long-Term; Eutrophication

Abstract| Introduction| Samples Collection and Analysis| Results and analysis| Results and Results and Discussion| Conclusion| Acknowledgments| References|