Multi Epitope Vaccine Candidate against Mycobacterium Tuberculosis

Volume 3 - Issue 2

Mostafa Norizadeh Tazehkand¹* and Orkideh Hajipour²

Received:November 04, 2019;   Published:November 15, 2019

DOI: 10.32474/DDIPIJ.2018.03.000160

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Abstract

Tuberculosis or TB is a disease caused by a type of bacteria called Mycobacterium tuberculosis that mainly affects the lungs, even though other tissues and organs may be involved. The present study aims to choose and analyze different epitopes of Apa, PstS3, SecA2, and RD1 using bioinformatics analysis and software. After that molecular docking assay was used to recognize the binding energy and affinity of designed epitopes to HLA-A0201. The vaxiJen score of these sequences were 0.5233, 0.8066, 0.5008, and 0.5512 respectively. B-cell epitopes were predicted using IEDB and Vaxign webserver was used to prediction of binding of peptides to MHC class-I. Physicochemical analysis of vaccine showed that the molecular weight of candidate vaccine is 54.331kDa with the half-life of 30 hours in mammalian reticulocytes, greater than 20 hours for yeast, and greater than 10 hours for Escherichia coli. The chemical formula of vaccine was C₂₄₁₀H₃₇₅₀N₆₄₈O₇₄₇S₁₈ and theoretical pI of candidate vaccine was 5.85. There are 496 amino acids in the protein structure of which 67 residues with negative charge (Asp + Glu) and 63 (Arg + Lys) residues are negative charge. The instability index, aliphatic index, and grand average of hydropathicity of candidate vaccine were 27.44, 67.46, and -0.521 respectively. According to the result of protparam and pepcalc analysis, the vaccine is soluble in water. Parabi analysis showed that the membrane helices value of vaccine was 25.2%. The vaccine does not have more transmembrane helix, therefore no expression difficulties are predicted in the production of vaccine. 3Drefine was used to minimize and correct the hypothetical structure. Ramachandran plot analysis by procheck showed that 96.3% of residues are in most favored regions, 3.7% of residues are additional allowed regions and 0% of residues is in disallowed regions. Molecular docking result of this research revealed that our designed vaccine can stimulate HLA0210. The vaccine may activate humoral and cellular immune responses against of Mycobacterium tuberculosis. However, the vaccine could be cloned and expressed in different host cells.

Keywords:Moringa oleifera; Tamarindus Indica Seeds

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