Tongluo Decoction Blocks Apoptotic Mitochondrial Pathway and Inhibits Cardiomyocyte Apoptosis of Coronary Microembolization Rats Volume 2 - Issue 3

Xuefeng Li¹*, Junnan Zhao^1,2, Jiaying Zhang¹, Yanbing Shi¹, Yinghuan Dou¹, Jing Zhou¹, Yang Luo¹, Yan Zhu³, Yang Yuan¹, Ying Wang¹, Mengru Zhou¹ and Miao Li¹

• ¹Lanzhou University, school of Basic medical sciences, Lanzhou, China
• ²Xiangyang Hospital of traditional Chinese medicine, Xiangyang, China
• ³Gansu Provincial hospital, Lanzhou, China

Received: February 02, 2020;   Published: April 20, 2020

*Corresponding author: Xuefeng Li, Lanzhou University, school of Basic medical sciences, Lanzhou, China

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Abstract

Background: We found that Tongluo Decoction has had good protection for myocardial cells injury of CME rats. But it is not clear whether Tongluo Decoction has inhibitory effect on myocardial apoptosis. So we want to study the effect and mechanism of Tongluo decoction to cardiomyocyte apoptosis following coronary microembolization in rats. Methods: 46 male Wistar rats were randomly divided into blank group (n=6), model group (n=10), Tongluo Decoction low dose group (n=10), Tongluo Decoction high dose group (n=10) and Tongxinluo group (n=10). In addition to the blank group, the rats of other groups were established the model of autologous thromboembolism microembolism by interventional method. The low dose group of Tongluo Decoction was given 7.8g/kg for gavaging, the high dose group of Tongluo Decoction was given 23.4g/kg, Tongxinluo group was given 0.2g/kg, once-a-day, for 12 day. The judgment of the model was done with HE and HBFP staining, the myocardial apoptosis rate was observed by TUNEL staining. Western Blot was used to detect Bcl-2, Bax and Caspase-3 in cardiac myocytes.

Results: The microembolization vessel count ratio, ischemic myocardial area ratio and the apoptosis rate of myocardial cells in model group were significantly higher than that in blank group, bcl-2 and bcl-2/bax decreased, and bax and caspase-3 were significantly increased (P < 0.05).Compared with model group, Tongluo Decoction low dose group, Tongluo Decoction high dose group and Tongxinluo group microembolization vessel count ratioischemic myocardial area ratio apoptosis rate decreased (P < 0.05), bcl-2, bcl-2/bax increased, Bax and caspase-3 decreased (P < 0.05). Compared with Tongxinluo group, the ratio of ischemic myocardial area, microembolization vessel count ratio, apoptosis rate, the expression of bax and caspase-3 proteins decreased (P > 0.05), bcl-2 and bcl-2/bax ratio increased in Tongluo Decoction high dose group (P > 0.05). bcl-2 and bcl-2/bax ratio increased obviously in Tongluo Decoction low dose group (P < 0.05).

Conclusion: Tongluo Decoction can inhibit cardiomyocyte apoptosis following coronary microembolization in rats, and its possible mechanism is related to blocking mitochondrial apoptotic pathway.

Keywords: Tongluodecoction; Rat; Coronary microembolization; Cardiomyocyte apoptosis

Abbrivations: Percutaneous coronary intervention (PCI); Coronary microembolization (CME); Hematoxylin-Eosin staining (HE); Hematoxylin basic fuchsin picric staining (HBFP); Terminal deoxynucleotidyl transferase-mediated dUTP Nick End-Labeling (TUNEL); High performance liquid chromatography (HPLC); Tongluo decoction (TLD); Optical density (OD); Bcl-2 Assaciated X protein (Bax); Cysteinyl aspartate specific protease-3 (Caspase-3); B-cell lymphoma-2 (Bcl-2)

Abstract| Introduction Methods Results Discussion Conclusion References