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ISSN: 2641-1725

LOJ Medical Sciences

Research Article(ISSN: 2641-1725)

Evaluation of Antioxidant Activity of The Consciousness Energy Healing Treated Novel Proprietary Formulation after Oral Administration in Male Sprague Dawley Rats Volume 3 - Issue 2

Mahindra Kumar Trivedi1 and Snehasis Jana2*

  • 1Trivedi Global, Inc., Henderson, Nevada, USA
  • 2Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India

Received: March 27, 2019 ;   Published:April 03, 2019

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India

DOI: 10.32474/LOJMS.2019.03.000160

Abstract PDF


The aim of this study was to evaluate the antioxidant and immunomodulatory activity of Consciousness Energy Healing Treated test formulation using antioxidants, hematology and biochemical parameters in male Sprague Dawley rats. A proprietary test herbomineral formulation was prepared and divided into two parts, one was represented as control, while the other was treated with Biofield Energy Treatment by Mr. Mahendra Kumar Trivedi (known as The Trivedi Effect®) and defined as the Biofield Energy Treated sample. The level of malondialdehyde (MDA) was significantly reduced by 11.56%, 30.13%, 24.28%, 38.03%, and 40% in the Biofield Energy Treated test formulation group (G5), Biofield Energy Treated animals per se at day -15 (G6), Biofield Energy Treated test formulation at day -15 (G7), Biofield Energy Treated animals + Biofield Energy Treated test formulation at day -15 (G8) and Biofield Energy Treatment per se to animals plus untreated test formulation (G9), respectively compared to the stress control group (G2). Myeloperoxidase (MPO) was significant reduced by 10.72% in the G5 group compared to the untreated test formulation group (G4). Glutathione (GSH) was significantly increased by 13.69%, 35.71%, and 18.08% in the G4, G5, and G7 groups, respectively than G2. Glutathione peroxidase (GPx) was significantly increased by 97.95%, 97.59%, 98.00%, and 98.14% compared to the G2. Total leukocyte count (TLC) was significantly increased by 19.55%, 34.92%, 45.39%, 41.91%, and 20.25% in the G5, G6, G7, G8, and G9 groups, respectively compared to the G4. Neutrophil count was increased by 13.26% and 11.07% in the G6 and G7 groups, respectively compared to the G4. Triglyceride and very low density lipoprotein (VLDL) were reduced by 24.75% and 24.91%, respectively in the G9 group compared to the G4 group. Creatine kinase - myocardium band (CK-MB) in heart was significantly reduced by 13.91% in the G9 group compared to the G2. Magnesium was increased significantly by 20.33%, 18.42%, 18.05%, 29.32%, 34.59%, and 15.04% in the G4, G5, G6, G7, G8, and G9 groups, respectively compared to the G2. Uric acid was significantly reduced by 34.25% in the G7 group compared to the G4. Besides, feed consumption, body weight, organ to body weight ratio, and histopathology examination did not show any alteration, indicated that Biofield Energy Treated test formulation was found be safe. Overall, data suggested that Biofield Energy Treatment per se and Biofield Energy Treated test formulation have shown significant anti-stress activity and could be utilized in various stress-related disorders like depression and anxiety, acute stress disorder (ASD) and posttraumatic stress disorder (PTSD), sleep difficulties, ADD, ADHD, social avoidance, irritability, including asthma, obesity, diabetes, headaches, gastrointestinal problems, Alzheimer’s disease, accelerated aging, and premature death.

Keywords: Herbomineral formulation; Biofield Energy Healing; The Trivedi Effect®; Antioxidant; Hematology; Biochemistry; Relative organ weight; Histopathology


Dysfunction of an immune system leads to various diseases viz. arthritis, ulcerative colitis, asthma, allergy, parasitic diseases, cancer, and infectious diseases. Reduction in the number of the neutrophils and macrophages and phagocytic function is responsible for the suppression of immune system and allows opportunistic pathogens to overwhelm the host to cause secondary infection [1,2]. Antioxidant activity refers to the ability of a compound to delay, inhibit or prevent the oxidation by scavenging the free radicals that leads to reduce oxidative stress. While, oxidative stress has a vital role in the etiology of several diseases and condition such as diabetes, cardiovascular disease, inflammatory condition, cancer, aging, etc. The antioxidant may prevent diseases by scavenging the free radicals, inhibiting lipid peroxidation and by many other mechanisms [3]. In this aspect, authors designed this experiment and formulated the test formulation, which was a combination of trace elements such as zinc chloride, magnesium gluconate hydrate, sodium selenate, Iron (II) sulfate, nanocurcumin, copper chloride, ascorbic acid, and cholecalciferol. Each ingredient of this formulation already been used as nutraceutical supplement [4- 7]. The use of an alternative system of medicine for any types of ailments resulted in an intense area of research and discovery of many herbs and minerals with their potential to cure diseases [8]. The complementary and alternative medicine (CAM) therapy has been increased nowadays for both therapeutic and prophylactic management of various chronic disorders in the human population [9].

Various scientific data reported the positive impact of Biofield Energy Treatment on immune systems [10,11]. The National Center for Complementary and Alternative Medicine (NCCAM) has accepted that by utilizing energy field as Energy Therapies. The Trivedi Effect® - Biofield Energy Healing Therapy already has been popularized to improve the potential health in different group of population benefits. It improved the overall productivity of crops in the field of agriculture [12,13], positive impact on cancer cells [14], altered characteristics features of microbes in the field of microbiology [15, 16], structural, physical, and thermal properties of several metals and ceramics [17,18], improved immunomodulatory activity in in vitro assay [19,20], enhanced skin protective activity [21,22] and improved excellent outcomes of various nutraceutical compounds in the fields of nutraceuticals [23,24] and biotechnology [25,26]. In this study, the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the test formulation was explored in term of immunomodulatory activity, which might be used as a better immunomodulatory function. Therefore, the objective of this experiment was to evaluate the effect of the Biofield Energy Treated and untreated test formulation concerning to antioxidant activity, hematological, biochemical, body weight, feed consumption, and histopathology parameters.

Materials and Methods


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