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ISSN: 2641-6921

Modern Approaches on Material Science

Research Article(ISSN: 2641-6921)

Application of Biodegradable PLGA-PEG-PLGA/CPC Composite Bone Cement in the Treatment of Osteoporosis Volume 4 - Issue 2

Chao Guo, Dongyang Niu, Jia Liu, Xiaogang Bao and Guohua Xu*

  • Department of Orthopedic Surgery, Spine Center, Changzheng Hospital, Second Military Medical, University, China

Received:March 29, 2021;   Published: June 07, 2021

*Corresponding author: Guohua Xu, Department of Orthopedic Surgery, Spine Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China


DOI: 10.32474/MAMS.2021.04.000184

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Abstract

Objective: To evaluate the biological activity, safety and effectiveness of poly (lactic acid), poly (glycolic acid) and poly (ethylene glycol) / calcium phosphate cement (PLGA-PEG-PLGA/CPC) in vivo and in vitro.

Methods: The PLGA-PEG-PLGA/CPC composite bone cement was used to interact with MC3T3-E1 mouse osteoblasts in vitro, and the in vitro compatibility of the bone cement was tested by CCK8 experiment. Alizarin red staining and alkaline phosphatase activity were used Detect the osteogenic properties of the bone cement. Twenty healthy female New Zealand rabbits were selected for the establishment of ovariectomized osteoporosis model, and imaging and histomorphology were used to evaluate.10 rabbits used 30wt.% PLGA-PEG-PLGA/CPC composite bone cement with good physical and chemical properties and in vitro activity to be implanted into the lumbar vertebral body as the experimental group, and 10 rabbits used polymethyl methacrylate (PMMA) bone cement as the experimental group. Control group. The imaging and histomorphological results of the vertebral body after 12w of implantation were studied. Micro CT examination and hematoxylin-eosin (HE) staining were used to observe the distribution of bone cement, bone cement degradation and regeneration Bone tissue.

Results: In vitro biological activity evaluation: CCK-8 colorimetric method was used to detect cell proliferation. The results showed that the primary osteoblasts proliferated significantly in the PLGA-PEG-PLGA/CPC composite bone cement extract, with clear osteoinductive properties and promotion of osteoblasts The function of cell proliferation. Animal experiment evaluation: The results of the rabbit PVP experiment found that 30wt.% PLGA-PEG-PLGA/CPC composite bone cement has good operability and can completely fill the defect site. Postoperative Micro-CT and histological examination showed that the composite bone cement material was evenly distributed in the vertebral body, and a small amount penetrated into the trabecular bone to achieve a sufficient filling effect. After 12w, a small amount of CPC degradation and absorption and the formation of surrounding new bone matrix can be seen, and the formation of callus around the vertebral body is obvious.

Conclusion: PLGA-PEG-PLGA/CPC composite bone cement has the biological activity of promoting the proliferation of osteoblasts. Through animal PVP experiments, 30wt.% PLGA-PEG-PLGA/CPC composite bone cement has good maneuverability, and its good biodegradability and osteoinductive effect contribute to the formation of surrounding new bone trabeculae.

Abstract| Introduction| Methods and Materials| Statistics| Results| Discussion| Acknowledgement| Conflict of Interests| References|

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