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ISSN: 2641-6794

Received: January 20, 2020;   Published: January 28, 2020

DOI: 10.32474/OAJESS.2020.04.000191

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Abstract

A novel technique was used to transform and utilize selenate/selenite in soil. Tea seedlings ontainingHerbaspirillum sp.strain WT00F were cultivated and then transplanted in leniferous soils. The grown tea plants not only effectively reduced the level of selenate/selenite in ferous soils but also enhanced selenium enrichment in tea leaves.

Introduction: As a nonmetallic element, selenium (Se) is a member of the chalcogens. In the nature, Se usually occurs in organic and inorganic forms. Its organic form (e.g. selenocysteine and selenoproteins, etc) mainly presents in living organisms where the organic selenocompounds can be metabolized [1,2], whereas its inorganic form (e.g. selenate and selenite oxoanions) primarily exists in natural environments. Selenate (SeO₄2^-) and selenite (SeO₃2^-) are water soluble so that they have potential mobility and bioavailability in the environment [3]. At low dosage, Se stimulates the growth of the plant whereas at high dosages it causes plant damage [4-6]. The deficiency of Se has been thought to be associated with over 40 human diseases [7,8] but the excessive intake of Se seriously damages human health [9]. Although the distribution area of Se is limited, Se-pollution in the surrounding land of selenium mining area is quite serious. Soluble Se⁶⁺ and Se⁴⁺ can be reduced to insoluble non-toxic elemental selenium (Seo) by microbes. The reduction of selenate/selenite to elemental selenium by microbes is an effective way to remove them from contaminated soil, water and drainage [10]. Herbaspirillum sp. strain WT00F is an endophytic bacterium isolated from tea plant (Camellia sinensis L) [11]. It enters plants via wound infection and only colonizes in Camellia plants as a specialist. Like Herbaspirillum sp. strain WT00C [12], this bacterium has a strong capability of reducing selenate and selenite to form red elemental selenium (Seo) or selenoproteins. Moreover, this bacterium also stimulates lateral root formation and bud growth of tea cuttings once it enters tea pole via the incision at the end of tea cuttings [13]. Thus, we attempted to use this bacterium to promote tea plants to transform and utilize selenate/selenite in soil and found that the tea plants colonized by Herbaspirillum sp. strain WT00F effectively utilized selenate/selenite in soil and enriched selenium in tea leaves.

Abstract| Technological Scheme| Experimental Effects and Discussion| Acknowledgement| References|