Comparative Wound Healing Effects of Honey, Olive Oil, Pawpaw Fruit Extract and Iodine in Diabetic Rats: an Evaluation and Prioritization of Potential Alternative Therapeutic Options Volume 2 - Issue 5

Magnus Michael Chukwudike Anyakudo* and Oluwabukola Mary Ayibiowu

• Endocrinology/Metabolism and Clinical Nutrition Research Unit of Department of Physiology, University of Medical Sciences, Nigeria

Received: January 27, 2020   Published: February 11, 2020

Corresponding author: Magnus Michael Chukwudike Anyakudo, Endocrinology/Metabolism and Clinical Nutrition Research Unit of Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, P.M.B 536, Ondo City, Ondo State, Nigeria

DOI: 10.32474/ADO.2020.02.000146

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Abstract

High cost of conventional diabetic wound management is of public interest due to their social and economic burden on the individuals and care givers. This experimentally-controlled designed study compared the wound healing potentials of natural products: Honey, Olive oil, Pawpaw fruit (unripe mesocarp) extract (PFE) and Iodine (positive control) in diabetic rats. Twenty-eight male Wistar rats categorized into four groups (n = 7, each) weighing between 150 - 200g were used for this study which lasted four weeks. A full thickness excision wound of circular area 300 mm2 and 2 mm in-depth was inflicted on all rats after light anesthesia with i.v. ketamine (90mg/kg b.w.). Aliquot portions of Olive oil, Honey, Iodine and PFE were topically applied to the opened wounds twice a day. Wound areas were measured on days 1, 7, 14, 21 and 28 using a transparent sheet and a permanent marker. Wound healing activity was assessed using percentage area of wound contraction, epithelization period and granulation tissue integrity. Data were analyzed using Microsoft Excel and SPSS v. 22. Comparison between groups was made using one way ANOVA. P values < 0.05 were considered significant. Significant wound contraction occurred on 14th day of treatment in all groups: Iodine (99.4%), Olive oil (98.4%), Honey (92.4%) and PFE (90.2%) with different remarkable epithelization periods (days): Iodine (14), Olive oil (15), Honey (16) and Pawpaw extract (17). Granulation tissue integrity improved respectively in the order of PFE, Honey, Olive oil and Iodine. The results of this study revealed that honey, olive oil and PFE as natural products have varying beneficial effects on wound healing process in diabetic rats.

Keywords: Natural Products; Wound Healing; Diabetic Rats; Alternative Therapeutic Options

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