The aim of this article is to highlight innovative scientific research that deserves attention about ozone treatment to overcome livestock infertility problems. Reproductive efficiency is essential for sustainable dairy and meat productions and failures in reproduction lead to economic losses. There are many challenges affecting fertility in farm animals. Oxidative stress and microbial infections during reproductive cycle of cattle are important causes of decreasing fertility and is considered an important challenge affecting reproduction. These challenges affect the animal during pregnancy, during or after parturition. The risks of physical damage during the birth process or at failure to release the placenta after parturition are often leading to increasing of microbial infections in the livestock.