Cellular Technologies (In Vitro Production of Embryos,
Germplasm Preservation and Somatic Reprogramming)
for Wisent (European Bison) Gene Pool Conservation
	 Volume 3 - Issue 5
		
		Anna M Duszewska1*, Magdalena Baraniewicz-Kołek1, Wojciech Bielecki2, Wojciech Niżański3, Agnieszka Partyka3,
Agnieszka Bernat4, Ajna Bihorac5 and Wanda Olech6
		
		
		
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- 1Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland
- 2Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
- 3Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life
Sciences, Poland
- 4Intercollegiate Faculty of Biotechnology, University of Gdansk & Medical University of Gdansk, Institute of Genetics and Animal
Biotechnology PAS, Poland
- 5Laboratory of Experimental Embryology, Institute of Genetics and Animal Biotechnology PAS, Poland
- 6Department of Genetics and Animal Breeding, Faculty of Animal Science, Warsaw University of Life Sciences, Poland
 *Corresponding author:  Anna M Duszewska - Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw
University of Life Sciences, Warsaw, Poland
				 
 
 
 
 
			
			
			
				Received: May 29, 2020;   Published: June 05, 2020
             
 DOI:  10.32474/CDVS.2020.03.000172
 
			 
			   		
			   
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		Abstract
Cellular technologies are a novel approach used for the protection of endangered animals, based on advances in cell biology and
biotechnology. In vitro production of embryos, germplasm cryopreservation and somatic reprogramming give new perspectives
for the protection of the European bison - Wisent (Bison bonasus) considered an endangered species. These biotechniques enable
the preservation of germplasm, i.e. genetic material in the form of mature and immature oocytes, spermatozoa, embryos, and also
somatic cells, which can be reprogrammed. They can contribute to the program of wisent restitution, particularly in the field of
preservation of genetic diversity, providing the reservoir of the gene pool for the future
 Keywords: Wisent - European bison (Bison bonasus), IVP, cryopreservation, gametes, embryos, iPSCs
   
 
        
       
		
				    
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