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ISSN: 2637-4609

Archives of Organic and Inorganic Chemical Sciences

Review Article(ISSN: 2637-4609)

Experimental Approach, Computational DFT Investigation and a Biological Activity in the Study of an Organic Heterocyclic Compound Volume 4 - Issue 1

Tribak Z*

  • Laboratory of Applied Chemistry, Sidi Mohamed Ben Abdellah University, Morocco

Received: January 10, 2019;   Published: January 24, 2019

*Corresponding author:Tribak Z, Laboratory of Applied Chemistry, Faculty of Sciences and Technology of Fez, Sidi Mohamed Ben Abdellah University, B.P. 2202-Road Imouzzer Fez Morocco

DOI: 10.32474/AOICS.2019.03.000177

Abstract PDF

Abstract

The title compound TZ1 was synthesized by N-alkylation reaction, and its structure was confirmed by 1H NMR, 13C NMR and IR, it was screened for their in vitro antibacterial activity by the agar well diffusion method against four bacteria, Gram-positive (Bacillus cereus, Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa). The molecule was studied with the density functional theory (DFT) at B3LYP/6–31G (d,p) level in order to determine the relationship between the molecular structure and the antibacterial inhibition behavior. The molecular geometry, frontier molecular orbitals and Mulliken atomic charge of the compound are investigated to get a better insight of the molecular properties. The molecular electrostatic potential (MEP) for a compound was determined to check their electrophilic or nucleophilic reactivity. The theoretical parameters offer significant assistance to understand the antibacterial inhibition mechanism indicated by the molecule and are in full agreement with the experimental results.

Keywords:5-Chlorosatin derivatives; N-alkylation reaction; antibacterial activity; DFT; Molecular electrostatic potential

Graphical abstract:

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Introduction

The 5-Chloroisatin is well documented as an important heterocyclic compound in the field of medicinal chemistry. My recently published book and review [1,2] contain a special chapter, dedicated to the chemistry of 5-Chloroisatin and their derivatives.

The 5-chloro-1H-indole-2 3-dione structure is a heterocyclic compound which easily participates in chemical reactions. Its bonding sites are analogous to pyrrole. As shown in Scheme 1, 5-Chloroisatin is reactive at four different positions including the carbon atom 3, nitrogen atom 1, the C2–C3 p-bond and the C2–N sigma bond.

This moiety of 5-Chloroisatin and their derivatives possess pharmacological and chemotherapeutic properties such as anti- cancer [3], anti-diabetic [4], anti-inflammatory [5], anti-malarial [6], anti-bacterial [7], anti-fungal [8], anti-viral [9] and others drugs for treatment of several diseases [10].

Density functional theory (DFT) has become a convenient method to decipher experimental results, in antibacterial activity; this technique makes it possible to accurately predict the inhibition efficiency of organic compounds on the basis of electronic and molecular properties as well as reactivity indexes [11]. The objective of current study is to explore relationship amongst structure and electronic properties of the synthesized 5-chloro-1- (2- (dimethylamino) ethyl) indoline-2,3-dione (TZ1) using DFT. Then, the evaluation of its antibacterial activity. (Scheme1)

Scheme 1:

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Experimental Details

Chemistry

Melting points were determined using the kofler bench apparatus and uncorrected. The spectra of 1H NMR spectra were recorded in CDCl3 on the Brucker Avance-300 spectrometer, operating at 300MHz and at 75MHz for 13C-NMR using TMS as an internal standard and Spin resonances are reported as chemical shifts (d) in parts per million (ppm). Infrared Spectra were run on AVATAR 320 AEK0200713 spectrometer and frequencies are reported in cm-1. The purity of the synthesized compound was confirmed by thin layer chromatography (TLC), performed on Silica gel 60 coated plates. UV light was used for the visualization of TLC spots [12].

General procedure

The 5-Chloroisatin derivatives TZ1 was prepared by mixing 0.2g (1.1 mmol) of 5-chloro-1H-indole-2,3-dione, (0.23g, 1.16mmol) of potassium carbonate in 15mL of N-N dimethylformamide (DMF) and (0.035g, 0.10mmol) of BTBA, then, the reagent is slowly added, the mixture is left at room temperature for 48 hours. The reaction mixture was concentrated by using rota vapor. The solid was separated out by filtration. It was carefully checked by thin layer chromatography. The compound was isolated by column chromatography by using different fractions of n-hexane and ethyl acetate [13-16].

Yield=89%; mp: 114 °C ; 1H NMR (CDCl3) δppm 7.53-7.54 (m, H, HAr); 7.51 (d, H, HAr, J=9Hz); 6.90 (d, H, HAr, J=9Hz); 3.85 (t, 2H, CH2, J=9Hz); 3.75 (t, 2H, CH2, J=9Hz); 2.15 (m, 6H, CH3). 13C NMR (CDCl3) δppm: 184.59 (C=O); 164.45 (N-C=O); 146.22, 141.13, 110.39 (Cq); 138.59, 126.08, 113.36 (CHAr); 55.90, 46.79 (CH2); 45.09 (CH3). Infra Red (KBr) cm-1: 3565, 3174, 30815 (C-H), 2975, 1720 (C=O), 1607 (NC=O), 1445, 1472 (C=C) 1185,1123 (N-C), 654 (C-Cl).

Antibacterial screening

Synthesized compound TZ1 was screened for their antibacterial activity against two Gram positive (Bacillus cereus and Staphylococcus aureus) and two Gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria by the agar well diffusion method, using LB medium (Luria Bertani medium: yeast extract 5.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1000mL). This technique was recommended by CLSI [17].

A sterile paper disk was placed on the surface of each plate and impregnated with 5μL of the TZ1 solution at a final concentration of 10mg/mL. Then, the plates were incubated at 4 °C for 2 hours to permit good diffusion before incubation at 37±2 °C for 24 hours. The diameters of the inhibition zones were measured in mm with the caliper. A disc impregnated with 2% dimethylsulfoxide as a negative control was made the experiment was carried out in triplicate.

In order to determine the Minimum inhibitory concentration (MIC) values, we started by the dilution of the TZ1 was prepared in a Mueller Hinton broth supplemented with bacteriological agar, to reach a final concentration between 5mg/mL and 0.004mg/ mL, 50μL of bacterial inoculum was added to each well at a final concentration of 106CFU/mL. DMSO (2%) was used as a negative control. The final concentration of our product was between 5mg mL-1 (3rd well) and 0.019mg mL-1 (well 11). Plates were incubated at 37 °C for 24 hours. After 2 hours of a subsequent incubation, bacterial growth was revealed by reduction of blue dye resazurin to pink resorufin [18].

Including, the minimum bactericidal concentrations (MBC) which is the last step in the protocol, a bactericidal control is carried out 24 hours earlier by streaking on a platelet agar, after microdilution to the broth by spreading 5μL of the negative wells on Luria Bertani agar plates.

Theoretical calculations

The computational studies of compound TZ1 were performed using the GAUSSIAN 09W [19] program package and visualized with the Gauss View on a personal computer using density functional theory (DFT) method with 6−31G (d,p) as the basis set [20]. The using HOMO and LUMO orbital energies, the ionization energy and electron affinity can be expressed as: IP = -EHOMO, EA = -ELUMO, respectively. The total hardness, η and electronegativity χ were given by the following relations: [21]. These DFT molecular parameters, and the equations used for their calculations are presented in the Table 1.

Table 1: Antibacterial activity of 5-chloro-1-(2-(dimethylamino) ethyl) indoline-2,3-dione.

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Result and Discussion

Synthesis of 5-chloro-1-(2-(dimethylamino) ethyl) indoline- 2,3-dione (TZ1) is outlined in Scheme 2, it was prepared according to a similar previous procedure [22]. 5-chloroisatin was used as a starting material for the synthesis of various substituted indole derivatives [23-27].

The (TZ1) was synthesized by the N-alkylation reaction of 5-chloro-1H-indole-2,3-dione in DMF, a base K2CO3 and a TBAB catalyst was added to a stirred solution at room temperature, Chloro-N,N-dimethylethanamine was added dropwise to the mixture under conditions of catalysis by phase transfer for 48 hours, the reaction was monitored by thin layer chromatography. After this time, the mixture was filtered and concentrated and dried under vacuum to afford the required product. The complex was obtained in good yield, stable in air, and is colored solid, soluble in methanol, chloroform, DMF, and DMSO.

The 1H-NMR, 13C-NMR and IR were used to assign the structure of synthesized compound. (Scheme 2)

Scheme 2: .

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Antibacterial activity

The In vitro antimicrobial screening tests of synthesized compound TZ1 was carried out as an antibacterial activity. the tested compound showed biological activity against different types of Gram-positive (Bacillus cereus and Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli), it showed zones of inhibition of MIC/MBC values ranging from 0.156/0.156 to 0.313/0.313mm against the Gram-positive bacteria and between 0.625/0.625 and 1.25/1.25mm against Gram-negative bacteria.

Coordination enhances the antibacterial activity and the TZ1 in the present study are more active against Gram-positive than Gram-negative bacteria [28]. On the other hand, it should also be noted that the presence of nitrogen and oxygen atoms which are the highest values of the negative charge on the molecule TZ1 suggesting that these centers have the maximum electron density and would preferentially interact with the micro-organisms Gram positive then increases the antibacterial potential.

Computational details

Frontier orbital energy analysis and other global reactivity descriptors: The all optimized structures along with the numbering scheme of TZ1 at DFT/B3LYP level using the 6-31+G (d,p) basis are shown in Figures 1-3.

Figure 1: Optimized molecular structure of TZ1 in ball and bond type format.

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Figure 2: Optimized molecular structure of TZ1 in tube format.

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Figure 3: Optimized molecular structure of TZ1 in wireframe format.

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The HOMO-LUMO orbitals help to characterize the chemical reactivity and kinetic stability of the molecule.

The analysis of the HOMO highlights the areas of the molecule that can donate electrons to electrophilic species while the analysis of the LUMO predicts the regions of the molecule with high affinity to accept electrons from nucleophilic species. The calculated HOMO–LUMO energy gap value is found to be 3.1673 eV (Table 2).

Table 2: Energetic parameters and Quantum chemical descriptors of TZ1.

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A molecule with a small frontier orbital gap (ΔEgap =3.1673 eV) is generally associated with a high chemical reactivity, low kinetic stability and is also termed as soft molecule [29]. The 3D plots of the frontier orbitals HOMO, LUMO for the TZ1 are shown in Figures 4 & 5.

Figure 4: Highest occupied molecular orbitals of TZ1.

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Figure 5: Lowest unoccupied molecular orbitals of TZ1.

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The dipole moment (μ(debye= 5.6982) tells about the polarity of the molecule. The higher value of dipole moment in case of TZ1 molecule is mainly attributed to an overall imbalance in charge from one side of a molecule to the other side is also evident from the MESP plot. DFT calculation gives an idea about the substance reactivity and site selectivity of the frameworks. By the computed value of HOMO and LUMO energy values for the TZ1, the electronegativity (χ), total hardness (η), Softness (σ), can be calculated. The significance of (η) and (σ) is to evaluate both the reactivity and stability [31].

Molecular electrostatic potential (esp) map: The molecular electrostatic potential mapped surfaces show the charge distributions of molecules three dimensionally which give clear and special signature of the interactions of the compounds [31].

The molecular electrostatic potential is related to the electronic density and a very useful descriptor for determining sites for electrophilic attack and nucleophilic reactions as well as hydrogenbonding interactions [32].

The MEP mapped surface of the compound TZ1 was calculated by DFT/B3LYP at 6 31G (d,p) basis set and MEP surface are plotted in Figure 6. Red, blue and green colors represent regions of the most electro negative, most electro positive electrostatic and zero potential, respectively [33].

Figure 6: Molecular electrostatic potential surface of compound TZ1

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Indeed, it is clear that in the compound TZ1 oxygen atom (red region) and nitro groups react with electrophilic sites, in all hydrogens atoms (blue region) react with nucleophilic sites.

Mulliken charges analysis

The Mulliken atomic charges have a significant role in the application of quantum chemical calculations to molecular systems, by determining the electron population of each atom as defined by the basis function [34]. Table 3 exhibits the calculated mulliken atomic charges except for atoms H by DFT/B3LYP at 6 31G (d,p) basis set. Also, the color range in the scale of positive and negative charge and graphical representation for Mulliken atomic charges of TZ1 is shown in Figure 7.

Table 3: Values of Mulliken charges at the atomic sites of TZ1.

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Figure 7: Mulliken atomic charge representation of TZ1.

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From the listed tabulated values (Table 3) of atomic charge, we can summary that the charge on the carbon atom (C6) is greater than other carbon atoms in the all compounds because it is connected to electronegative chloride atom (Cl10). Then, all nitrogen and oxygen atoms (N13, N22, O14 and O15) are the most negatively charged ones, suggesting that these centers have the maximum electron density, which can interact with the positively charged part of the receptor easily.

Conclusion

In summary, we report the synthesis and characterization of 5-chloro-1- (2- (dimethylamino) ethyl) indoline-2,3-dione (TZ1) in excellent yield. The antibacterial activity of TZ1 has been explored experimentally and by quantum calculations. The frontier orbital energy analysis, mulliken atomic charges and electrostatic potential were also studied by using the DFT method at B3LYP/6–31G (d,p). The antibacterial bioassay showed that it possessed excellent activity.

Acknowledgements

The author would like to thank all the people who helped to carry out this work such as 1H NMR, 13C NMR, IR, and for antibacterial activity.

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