Effects of the Consciousness Energy Healing Treatment on the Physicochemical and Thermal Properties of Zinc Chloride Volume 4 - Issue 1

Alice Branton¹ and Snehasis Jana²*

• ¹Trivedi Global, Inc., Henderson, USA
• ²Trivedi Science Research Laboratory Pvt. Ltd., India

Received: February 09, 2019;   Published: March 19, 2019

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

DOI: 10.32474/AOICS.2019.04.000178

Abstract PDF

Zinc chloride (ZnCl₂) is used as a common source of zinc supplement in the pharmaceutical and nutraceutical formulations. In this experiment, the impact of the Trivedi Effect®- Consciousness Energy Healing Treatment on the physicochemical, thermal, and behavioral properties of ZnCl₂ was evaluated using sophisticated analytical techniques. The test sample ZnCl₂ was divided into two parts. One part of ZnCl₂ was considered as a control sample (no Biofield Treatment was provided), while second part received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a famous Biofield Energy Healer, Alice Branton and termed as treated ZnCl₂. The powder XRD crystallite sizes and peak intensities of the treated ZnCl₂ were significantly altered ranging from -16.67% to 99.97% and -88.51% to 202.23%, respectively; however, the average crystallite size was significantly increased by 14.14% compared with the control sample. The particles size values of the treated ZnCl₂ at d₁₀, d₅₀, and d₉₀ and D (4,3) were significantly increased by 886.20%, 36.70%, 28.90%, and 92.71%, respectively compared to the control sample. Hence, the surface area of the treated ZnCl₂ was significantly decreased by 81.16% compared with the control ZnCl₂. The latent heat of fusion of the treated ZnCl₂ was significantly increased by 26.48% compared with the control sample. However, the latent heat of decomposition of the treated ZnCl₂ was significantly decreased by 85.20% compared with the control sample. The study results anticipated that the Trivedi Effect®-Consciousness Energy Healing Treatment might produce a new polymorphic form of ZnCl₂ with larger crystallite and particle size and reduced surface area, which would improve the flowability, shape, and appearance compared with the control sample. The Biofield Energy Treated ZnCl₂ would be very useful to design more efficacious nutraceutical or pharmaceutical formulations against cancer, inflammatory diseases, stress, immunological disorders, aging, Parakeratosis, dysosmia, anorexia, hypogeusia, hypogonadism, Wilson’s disease, etc.

Keywords: Zinc chloride; Consciousness energy healing treatment; The Trivedi Effect®, PXRD, Particle size; Surface area; DSC

Zinc is an essential trace element which regulates a broad spectrum of physiological functions in the human body including prenatal and postnatal development [1]. Zinc is plenty available in animal meat, fish, shellfish, fowl, eggs, milk, wheat (germ and bran) and various seeds like sesame, poppy, alfalfa, mustard, celery, beans, nuts, almonds, whole grains, pumpkin seeds, sunflower seeds, and blackcurrant [2,3]. It is found in more than 100 enzymes (i.e., carbonic anhydrase, carboxypeptidase, etc.) and part of the protein (i.e., metallothioneins), serves as structural ions in transcription factors, nucleic acid, and protein metabolism, coordinate amino acid chains, etc. [4-7]. Pharmacologically it acts as a fertility enhancing, retinoprotective, immunomodulatory, antioxidant, and putative antiviral in the body [6,8]. It has been reported with increased leukocyte count and phagocytic index in combination with Glycyrrhiza glabra [9]. Zinc is also used to prevent the progress of several deficiency disorders include Parakeratosis, hypogeusia, dysosmia, anorexia, hypogonadism, growth retardation, Wilson’s disease, etc. [10-13]. Deficiency of zinc is generally due to insufficient dietary intake, which can be associated with malabsorption, chronic liver and renal disease, acrodermatitis enteropathica, sickle cell disease, malignancy, diabetes, and other chronic illnesses [14].

Zinc chloride (ZnCl₂) is used as a common source for zinc in the pharmaceutical, nutraceutical, and cosmetic industry for the preparation of drug and diagnostic agent, mouth-wash, dentin desensitizer, deodorant, disinfectant, protein precipitator, and insulin preparation due to its powerful astringent and mild antiseptic properties [15-18]. The literature reported that ZnCl₂ could also be used in the treatment of skin cancer as a destructive agent and palliative treatment of canine and malignant skin wounds [19,20]. Therefore, ZnCl₂ was considered one of the components in the nutraceuticals/pharmaceuticals supplement for the prevention and treatment of various human disorders. ZnCl₂ also used in other industry dealing with chemicals, metallurgy, textile, and paper manufacture [17]. ZnCl₂ is irritant to skin, caustic to the gastrointestinal tract, occasionally leading to hematemesis, lethal to the human at the dose of 3-5 milligram, and extremely detrimental to the pulmonary and lungs on exposure to ZnCl₂ smoke [17,21].

Physicochemical properties of a nutraceutical and pharmaceutical compounds play a very important role in its therapeutic efficacy [22]. The Trivedi Effect®-Consciousness Energy Healing Treatment significantly altered the physicochemical and thermal properties of many pharmaceutical/nutraceutical compounds [23-25], along with improved the bioavailability of poorly bioavailable compounds (i.e., resveratrol, berberine, and 25-hydroxyvitamin D3 in male Sprague-Dawley rats) [15-17]. The Trivedi Effect® (the Biofield Energy Treatment) is a natural and only scientifically proven phenomenon in which a skilled individual can harness this inherently intelligent energy from the “Universe” and transmit it anywhere on the planet via the possible mediation of neutrinos [27-29]. The “Biofield” is infinite, paradimensional, and dynamic electromagnetic field is present surrounding the human body. The Biofield Energy can easily flow between the human and environment that leads to the uninterrupted movement or matter of energy [30-32]. The Biofield based Energy Healing Therapies also used against various human disease conditions and accepted worldwide. It has been recognized as a Complementary and Alternative Medicine (CAM) health care approach by National Center of Complementary and Integrative Health (NCCIH) along with other therapies, medicines and practices such as Ayurvedic medicine, traditional Chinese herbs and medicines, yoga, Reiki, hypnotherapy, aromatherapy, homeopathy, Qi Gong, Tai Chi, chiropractic/osteopathic manipulation, meditation, acupuncture, acupressure, naturopathy, healing touch, cranial sacral therapy, movement therapy, etc. [32-34]. The Biofield Energy Treatment has been reported in numerous peer-reviewed scientific journals with significant outcomes in medical research [35], microbiology [36,37], biotechnology [38,39], metals, ceramics [40,41], organic compounds [42], and agriculture [43]. The physicochemical properties of pharmaceutical/nutraceutical compounds have a vital role in bioavailability as well as the stability of the drug during manufacturing, storage, and packaging [22,44]. Thus, the study was designed to evaluate the impact of the Trivedi Effect®- Consciousness Energy Healing Treatment on the physicochemical and thermal properties ZnCl₂ using powder X-ray diffraction (PXRD), particle size distribution analysis (PSD), and differential scanning calorimetry (DSC) analytical techniques.

Chemicals and reagents

Melting points were determined using the kofler bench apparatus and uncorrected. The spectra of 1H NMR spectra were recorded in CDCl3 on the Brucker Avance-300 spectrometer, operating at 300MHz and at 75MHz for 13C-NMR using TMS as an internal standard and Spin resonances are reported as chemical shifts (d) in parts per million (ppm). Infrared Spectra were run on AVATAR 320 AEK0200713 spectrometer and frequencies are reported in cm-1. The purity of the synthesized compound was confirmed by thin layer chromatography (TLC), performed on Silica gel 60 coated plates. UV light was used for the visualization of TLC spots [12].

General procedure

The ZnCl₂ powder was procured from Tokyo Chemical Industry Co., Ltd. (TCI), Japan and other chemicals used in the course of the experiment were purchased in India.

The Trivedi Effect®-Consciousness Energy Healing Treatment

The test sample ZnCl₂ was equally divided into two parts. One part of the test sample did not receive Biofield Energy Treatment and was termed as the untreated or control ZnCl₂ sample. But, the control ZnCl₂ sample was subjected to a “sham” healer, under the same laboratory conditions; whereas, the “sham” healer totally ignorant about the Biofield Energy and its treatment procedure. The 2nd part of the test sample was exposed to the Trivedi Effect® - Consciousness Energy Healing Treatment (Biofield Energy Treatment) by a renowned Biofield Energy Healer, Alice Branton, USA, remotely under standard laboratory conditions for 3 minutes and known as Biofield Energy Treated ZnCl₂. After the treatment, both the samples were kept in similar sealed conditions and further characterized thoroughly using modern analytical techniques.

Characterization

The powder XRD analysis of ZnCl₂ powder sample was performed with the help of Rigaku Mini Flex-II Desktop X-ray diffractometer (Japan) [45,46]. The average size of crystallites of both the samples was calculated from powder XRD data using the Scherrer’s formula (1)

Where G is the crystallite size in nm, λ is the radiation wavelength, k is the equipment constant, β is the full-width at half maximum, and θ is the Bragg angle [47].

The PSA was performed using Malvern Mastersizer 2000, from the UK using the wet method. Similarly, the DSC analysis of ZnCl₂ samples was performed with the help of DSC Q200, TA instruments [45,46].

The % change in peak intensity, crystallite size, particle size, specific surface area, melting point, and latent heat of the Biofield Energy Treated ZnCl₂ sample was calculated compared with the control sample using the following equation 2:

Powder X-ray diffraction (PXRD) analysis

The PXRD diffractograms of both the control and the treated ZnCl₂ samples displayed very sharp and intense peaks in their respective diffractogram indicated that both the samples were crystalline nature (Figure 1). PXRD data such as Bragg angle (2θ), relative intensity (%), and crystallite size (G) for the control and Biofield Treated ZnCl₂ are presented in Table 1. The crystallite sizes of the Biofield Treated ZnCl₂ were significantly altered ranging from -16.67% to 99.97% compared with the control sample. However, the average crystallite size of the treated ZnCl₂ was significantly increased by 14.14% (Table 1, entry 37) compared to the control sample. The PXRD diffractograms of both the control and the treated ZnCl₂ displayed greater intensity at Bragg’s angle (2θ) near 25.6° (Table 1, entry 9). Besides this, the relative peak intensities of the PXRD peaks in the treated ZnCl₂ were significantly altered compared with the control sample (Table 1). Hence, the relative peak intensities of the Biofield Treated ZnCl₂ were significantly altered ranging from -88.51% to 202.23% compared to the control sample.

Table 1: Powder XRD data of the control and Biofield Energy Treated ZnCl₂.

Figure 1: PXRD diffractograms of the control and Biofield Energy Treated ZnCl₂.

From the results, it can be assumed that the crystal size, as well as the relative peak intensities, were significantly altered due to the Trivedi Effect®-Consciousness Energy Healing Treatment. As per the literature, any changes in the XRD patterns, such as crystallite size, and relative intensities, indicated the change of the crystal morphology as well as the proof of polymorphic transition [48- 50]. The crystal pattern, size, and even polymorphic form play an important role in the drug solubility, dissolution, and bioavailability of orally administered pharmaceutical and/or nutraceutical formulations [22]. Thus, the Biofield Energy Healing Treated ZnCl₂ could be very useful for designing a better and effective nutraceutical/pharmaceutical formulations.

Particle size distribution (PSD) analysis

The particle size values (d₁₀, d₅₀, and d₉₀, D (4, 3)) and the average surface area of the control and the treated ZnCl₂ were characterized and are presented in Table 2. The size of the particles in the treated ZnCl₂ sample at d₁₀, d₅₀, and d₉₀ and D (4, 3) were significantly increased by 886.20%, 36.70%, 28.90%, and 92.71%, respectively compared to the control sample. Hence, the surface area of the Biofield Energy Treated ZnCl₂ (110.00m²/g) was significantly decreased by 81.16% compared with the control sample (583.90m²/g).

Table 2: Particle size data {d₁₀, d₅₀, d₉₀ and D(4,3)} and surface area of the control and Biofield Treated ZnCl₂.

From the results, it can be assumed that the introduction of the external force like Biofield Energy Treatment (the Trivedi Effect®) transformed of the small ZnCl₂ particles into larger particles. Increase in the particle size enhances the flowability, improved product shape and appearance [51,52]. Therefore, Biofield Energy Healing Treated ZnCl₂ would have improved the flowability, shape, and appearance.

Differential scanning calorimetry (DSC) analysis

The DSC thermograms of the control and Biofield Energy Treated ZnCl₂ are presented in Figure 2. The thermal analysis data such as melting and decomposition temperature, and enthalpy of fusion and decomposition of the control and Biofield Energy Treated ZnCl₂ are presented in Table 3. The melting temperature of the Biofield Energy Treated ZnCl₂ (310.11 °C) was slightly decreased by 0.53% compared with the control sample (311.77 °C). Similarly, the decomposition temperature of the Biofield Energy Treated ZnCl₂ (382.96 °C) was decreased by 1.48% compared with the control sample (388.73 °C). The latent heat of fusion or enthalpy of fusion (ΔH_fusion) of the control and Biofield Energy Treated ZnCl₂ was 38.17 J/g and 48.28 J/g, respectively. The ΔH_fusion of the Biofield Energy Treated ZnCl₂ was significantly increased by 26.48% compared with the control sample. But the enthalpy of decomposition (ΔH_{decomposition}) of the control and Biofield Energy Treated ZnCl₂ were 208.60J/g and 30.87J/g, respectively. The ΔH_{decomposition} of the Biofield Energy Treated ZnCl₂ was significantly decreased by 85.20% compared with the control sample. Overall, the data indicated that Biofield Energy Treated ZnCl₂ needs less energy to undergo the process of melting after Biofield Energy Treatment.

Figure 2: DSC thermograms of the control and Biofield Energy Treated ZnCl₂.

Table 3: The latent heat of fusion/decomposition and melting/decomposition temperature values of the control and Biofield Energy Treated ZnCl₂.

T: melting/decomposition temperature, ΔH: Enthalpy of fusion/decomposition.

The experimental data revealed that the Trivedi Effect®- Consciousness Energy Healing Treatment had shown a considerable impact on the crystal, particle, thermal, and behavioral properties of ZnCl₂. The crystallite sizes of the Biofield Energy Treated ZnCl₂ were significantly altered ranging from -16.67% to 99.97% compared with the control ZnCl₂. Likewise, the relative peak intensities of the Biofield Energy Treated ZnCl₂ were significantly altered ranging from -88.51% to 202.23% compared to the control sample. However, the average crystallite size of the Biofield Energy Treated ZnCl₂ was significantly increased by 14.14% compared to the control ZnCl₂. The particle size values of the Biofield Energy Treated ZnCl₂ at d10, d50, and d90 and D (4, 3) were significantly increased by 886.20%, 36.70%, 28.90%, and 92.71%, respectively compared to the control sample. Hence, the surface area of the Biofield Energy Treated ZnCl₂ was significantly decreased by 81.16% compared with the control sample. The ΔH_fusion of the treated ZnCl₂ was significantly increased by 26.48% compared with the control sample. However, the ΔH_{decomposition} of the Biofield Energy Treated ZnCl₂ was significantly decreased by 85.20% compared with the control sample. The current study anticipated that the Trivedi Effect®-Consciousness Energy Healing Treatment might produce a new polymorphic form of ZnCl₂ with larger crystallite size and particle size would improve the flowability, shape, and appearance compared with the control sample. The Biofield Energy Treated ZnCl₂ would be very useful to design more efficacious nutraceutical or pharmaceutical formulations against cancer, inflammatory diseases, immunological disorders, stress, aging, Parakeratosis, hypogeusia, dysosmia, anorexia, hypogonadism, growth retardation, Wilson’s disease, etc.

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