Molecular Characterization of Chikungunya Virus in Serum- Relevance for Disease Management

Volume 2 - Issue 5

Komal Gupta^1,3, Vijay Kumar⁶, Ghanshyam⁴, Narotam Sharma*¹, Anita Rawat², Ruchi Agarwal^1,5, Shiny Saha¹ and Neelam Garg⁴

Received: April 25, 2018;   Published: May 03, 2018

DOI: 10.32474/AOICS.2018.02.000148

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Abstract

Background: Chikungunya Virus is a single stranded RNA virus of the genus alphavirus. The transmission of this virus occurs by the bite of infected mosquitoes. It is characterized by an abrupt onset of fever frequently accompanied by joint pain.

Aim: Molecular Characterization of Chikungunya Virus in Serum- Relevance for Disease Management.

Methods and Materials: 30 cases were considered having symptom of fever suspected to have chikungunya. RNA was isolated from magnetic beads methods which were further studied for Real Time-PCR (RT-PCR).

Results: Among the 30 cases suspected for chikungunya fever, only 18 were positive for the same. Hence, the prevalence was calculated to be 60%. About 39% of the positive cases were in the age range of 21-40 years, which is the active age group of the total positive cases, 66.66% were males and 33.33% were females. The virus can be even transmitted together as a co-infection. Patients infected with chikunguniya virus are more likely to experience symptoms of high fever, severe polyarthralgia and rash with lymphopenia.

Keywords: Chikunguniya; Real time polymerase chain reaction; Aedes aegypti; Aedes albopictus

Abbrevations: CHIK V: Chikungunya Virus; CMRL: Central Molecular Research Laboratory; SGRRIM & HS: Shri Guru Ram Rai Institute of Medical & Health Science; CDNA: Complementary DNA; MRNA: Messenger RNA

Abstract| Introduction| Materials and Methods| Results| Discussion and Conclusion| Acknowledgement| References|