The Anti-Inflammatory Activity of Ferulic Acid on NF-
κBDepends on Keap1
Volume 2 - Issue 2
Miriam Giacomarra, Annalisa Mangano and Giovanna Montana*
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- Istituto di Ricerca e Innovazione Biomedica, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy
*Corresponding author:
Giovanna Montana, Istituto di Ricerca e InnovazioneBiomedica, Consiglio Nazionale delleRicerche, Via Ugo
La Malfa 153, 90146 Palermo, Italy
Received: July 22, 2020; Published: August 04, 2020
DOI: 10.32474/LOJPCR.2020.02.000133
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Abstract
Nrf2 and NF-κB are the two master transcriptional factors activated by different cellular signals turned to counteract the
deleterious effects of pathological cellular processes linked to inflammation and oxidative stress.Several recent studies have
highlighted a molecular connection between NF-κB and Keap1/Nrf2 pathways. The Keap1 protein seems to be the central player in
this interaction, as it is involved in both IKKβ-NF-κB and Nrf2 modulation. Ferulic acid (FA) is a well-known antioxidant and antiinflammatory
agent, able to relieveinflammatory response viaNF-κB/IKK kinase, but until now the complete molecular network
under its action is not all clear. Immunoblot data conducted on LPS-treated macrophage-like RAW264.7 cells transfected with si-
Keap1 show that the FA anti-inflammatory and modulatory effects on NF-κB are abolished. Luciferase assay conducted in human
A549 cell line, in which Keap1 protein is partially inactive, highlights that NF-κB activation induced by LPS is refractory to FA
inhibition. This study proved that Keap1 and IKK together are important modulators of NF-κB and their activity is essential for FA
anti-inflammatory effectiveness.
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