Furosemide Diuretic Activity Evaluation in Female
Sprague-Dawley Rats
Volume 1 - Issue 4
Luis Castillo Henríquez1,2*, Andrea Campos Cerdas1,3 and Rebeca Chacón Durán1,4
-
Author Information
Open or Close
- 1Pharmacy Faculty, University of Costa Rica
- 2Phytopharmacology and Pharmaceutical Technology Laboratory (LAFITEC), Institute of Pharmaceutical Research (INIFAR), Costa Rica
- 3Regulatory Affairs, Calox, Costa Rica
- 4Pharmacy department, Coopesalud RL, Costa Rica
*Corresponding author:
Luis Castillo Henríquez, Phytopharmacology and Pharmaceutical Technology Laboratory (LAFITEC), Institute
of Pharmaceutical Research (INIFAR), San Jose, Costa Rica
Received: August 16, 2019; Published: August 22, 2019
DOI: 10.32474/LOJPCR.2019.01.000119
Full Text
PDF
To view the Full Article Peer-reviewed Article PDF
Abstract
This research article focuses on the evaluation of the diuretic activity of Furosemide administered to a group of female Sprague
Dawley rats, through the use of metabolic boxes.
Methods: A hydration volume of 5 mL/100 g was administered by cannulas for oral administration to group I (Furosemide) and
to group II (control). After 2 h the basal urine volume was determined. Group I was given 20 mg/kg of Furosemide and group II 20
mg/kg of physiological saline solution, both intraperitoneally. After the administration of the new hydration load of 5 mL/100 g, the
measurements of excreted urine volume and its pH were done every 30 minutes for 2 h.
Results: The maximum urinary excretion caused by this drug was achieved at 30 minutes post - administration, which shows a
statistically difference with respect to the control group. However, after 60 minutes it is observed that the diuretic activity begins
to decrease markedly due to the compensatory mechanisms of the physiology of the animal and the pharmacokinetics of the drug.
Conclussion: The rapid onset of action and brief effect showed by Furosemide is consistent with what is established in the literature.
Likewise, the slight decrease in post-treatment urinary pH with respect to the basal level, adjusts to the effect of Furosemide itself
and to the expansion effect of the urinary volume.
Keywords: Diuretic Activity; Furosemide; Intraperitoneal Administration; Loop Of Henle; Metabolic Boxes; Sprague-Dawley; Urine
Excretion
Abbreviations: ANP: Atrial Natriuretic Peptide; BNP: Brain Natriuretic Peptide; NKCC: Sodium-Potassium-Chlorine cotransporter;
SD: Sprague-Dawley
Abstract|
Introduction|
Materials and Methods|
Results|
Discussion|
Conclusion|
References|