Effects of Kav001 (A Kavain Analogue) on LPS-Induced Cytokines and Neutrophil Infiltration

Volume 1 - Issue 5

Xiaoren Tang¹*, Mansour Alasiri¹, Yinian Tang², Feng Cao¹ and Bing Liu³

Received: November 08, 2019;   Published: November 18, 2019

DOI: 10.32474/LOJPCR.2019.01.000122

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Abstract

Background: TNF-α, a cytokine, is known to be involved in LPS-induced inflammation. Our recent data indicate that treatment of Kavain in mouse or human cells significantly suppresses TNF-α production in response to LPS. In order to completely identify one Kavain analogue (Kav001) reported in our previous data, we further analyzed its biological function in this paper.

Methods and Results: Commercial Kavain or Kav001 as inhibitor was used. ELISA was performed for detection of TNF-α, IL-1β, IL-6, IL-4, or Caspase 1 after treatment of THP-1 cells with Kav001. Wild type mice (C57BL/6) were used for neutrophil infiltration assay. A kinase/protein array was performed to analyze if Kav001 affects any kinases/protein in response to LPS. Conclusions: It is clear that Kav001 not only inhibits TNF-α, but also IL-1β, IL-6, caspase 1 and neutrophil infiltration in response to LPS compared to control.

Abstract| Introduction| Materials and Methods| Compounds| Elisa| Neutrophil Infiltration Assay| Protein Array| Statistical Analysis| Results| Discussion| References|