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ISSN: 2644-1373

LOJ Pharmacology & Clinical Research

Research Article(ISSN: 2644-1373)

Effects of Kav001 (A Kavain Analogue) on LPS-Induced Cytokines and Neutrophil Infiltration

Volume 1 - Issue 5

Xiaoren Tang1*, Mansour Alasiri1, Yinian Tang2, Feng Cao1 and Bing Liu3

  • Author Information Open or Close
    • 1Department of Periodontology, Boston University Goldman School of Dental Medicine, USA
    • 2Boston University School of Medicine, USA
    • 3Department of General Dentistry, Boston University Goldman School of Dental Medicine, USA

    *Corresponding author:Xiaoren Tang, Department of Periodontology, Boston University Goldman School of Dental Medicine, 650 Albany Street, Boston, MA 02118, USA

Received: November 08, 2019;   Published: November 18, 2019

DOI: 10.32474/LOJPCR.2019.01.000122

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Background: TNF-α, a cytokine, is known to be involved in LPS-induced inflammation. Our recent data indicate that treatment of Kavain in mouse or human cells significantly suppresses TNF-α production in response to LPS. In order to completely identify one Kavain analogue (Kav001) reported in our previous data, we further analyzed its biological function in this paper.

Methods and Results: Commercial Kavain or Kav001 as inhibitor was used. ELISA was performed for detection of TNF-α, IL-1β, IL-6, IL-4, or Caspase 1 after treatment of THP-1 cells with Kav001. Wild type mice (C57BL/6) were used for neutrophil infiltration assay. A kinase/protein array was performed to analyze if Kav001 affects any kinases/protein in response to LPS. Conclusions: It is clear that Kav001 not only inhibits TNF-α, but also IL-1β, IL-6, caspase 1 and neutrophil infiltration in response to LPS compared to control.

Abstract| Introduction| Materials and Methods| Compounds| Elisa| Neutrophil Infiltration Assay| Protein Array| Statistical Analysis| Results| Discussion| References|