Glioblastoma Multiforme: a miPEP and miRNA Approach Volume 3 - Issue 1

Sepehr Saberian¹, Sharif Morsalin¹, Jinbo Fang¹, Christian M Mustroph², Seema Yousuf³, David A Gimbel², Veena N Rao¹ and E Shyam P Reddy¹*

• ¹Cancer Biology Program, Department of Ob/Gyn, Morehouse School of Medicine, Atlanta, GA,
• ²Department of Neurosurgery, Emory University, Atlanta, GA
• ³Department of Emergency Medicine, Emory University, Atlanta, GA

Received:March 04, 2022;   Published:March 28, 2022

Corresponding author: E Shyam P Reddy, Ph.D, Professor and Director, GCC Distinguished Cancer Scholar, Cancer Biology Program, Department of OB/GYN, Morehouse School of Medicine, Atlanta, GA, USA

DOI: 10.32474/LOJPCR.2022.03.000153

Abstract PDF

To view the Full Article   Peer-reviewed Article PDF

Abstract

Glioblastoma multiforme (GBM) is perhaps the most devastating tumor of the central nervous system (CNS), with an approximately 7% five-year survival rate. The micro-RNAs (miRNA) miRNA-200a and are downregulated in GBM. We hypothesize that miPEP-200a and miPEP-200b, discovered in our lab, has potential to restore levels of miRNA-200a and miRNA-200b and function as inhibitors of migration of glioma cells. For this work, a literature search was conducted, identifying various upregulated and downregulated members of the miRNA family in GBM. A search for the miPEP-200 family was also performed. An in-depth analysis of the blood-brain barrier (BBB) components was conducted to determine feasible candidates for therapeutic treatment of GBM. There has recently been a substantial increase in studies examining the role of miRNA in GBM. A thorough review of existing literature showed that in GBM, 73% (256) of miRNAs involved are upregulated and 27% (95) are downregulated. The latter group contains miRNA-200a and miRNA-200b. It’s been shown that miRNA has excellent stability in the blood stream. Although miRNA can cross the BBB from the cerebrospinal fluid (CSF) to the blood, the reverse movement has not yet been shown. Considering the characteristics of the BBB combined with experimental data from various studies showing the ability of miRNA to cross the BBB, miRNA has potential for use as a therapeutic agent in GBM. Specifically, miRNA-200a and miRNA-200b are known to be downregulated in GBM. Interestingly, our laboratory recently discovered the novel proteins miPEP-200a and miPEP-200b, both products of pri-miRNA translation. These proteins may demonstrate great potential for upregulating miRNA-200a and miRNA- 200b, given data suggesting this positive feedback mechanism found in one study. Since miPEP-200a and 200b were shown to inhibit migration of cancer cells and Epithelial to Mesenchymal Transition (EMT) of cancer cells, we propose that these miPEP-200a and miPEP-200b may function as therapeutic agents for targeting the migration of glioma cells.

Keywords:Gene Expression; Glioblastoma; glioblastoma multiforme; tumor; miRNA

Abstract| Introduction| Discussion| Conclusions| Acknowledgements| References|