Cellular Therapies in Onco-Hematology: 60 Years of Progress

In the last years, CAR-T cell therapy gained the attention of the entire scientific community thanks to their high rate of efficacy...


Introduction
In the last years, CAR-T cell therapy gained the attention of the entire scientific community thanks to their high rate of efficacy in some hematological and non-hematological malignancies.
However, the scientific bases of this innovative therapy should be traced back to the mid-90s, when animal models gave the first evidences concerning the protective role of T cell immunity against cancer. In 1956, the potential anti-tumor efficacy of bone marrow transplantation was suggested by results from murine models of leukemia treated with supralethal irradiation and subsequent marrow grafting [1]. However, the first clinical evidence about the anti-cancer activity of allogeneic bone marrow transplantation was given by Thomas E.D. who, in 1959, described the first successful marrow grafting in two leukemic patients who received supralethal irradiation and subsequent bone marrow transplantation by identical twin donors [2]. However, despite first descriptions of the Human Leukocyte Antigen (HLA) by [3,4], it took more than 10 years to successfully perform allogenic bone marrow transplantation from non-twins' donors. In 1961, in a two-page preliminary communication, the immunologist Jacques Miller demonstrated that early thymectomised mice developed a severe lymphocyte depletion and immune disfunction in adult age, thereby providing the first evidences about the role of thymus gland in normal T cell development and immune system function  [7]. Other evidences demonstrated that the overall survival (OS) of patients transplanted for malignant diseases developing mild cGVHD is better compared to patients without cGVHD. Even OS of patients with moderate cGVHD is not different from patients without cGVHD, as the slightly increased mortality associated with cGVHD is counterbalanced by lower disease-associated mortality [8]. According to these results, T cells [14]. Two years later, the immunologist Zelig Eshhar from the Weizman Institute in Israel, exploited the same technology to engineers T cells with the first chimeric molecule, produced by fusion of the antigen-binding portion of an antibody to the intracellular part of a T cell receptor. This new chimeric antigen receptor (CAR) became known as first-generation CARs. However, despite its technological innovation, these first-generation CARs did not persist in the body and were not clinically effective [15]. In order to overcome this issue, in 1998 Dr. Sadelain and his colleagues demonstrated that introducing a co-stimulatory molecule (e.g.

CD28) into the structure of CAR of engineered T cells allows them
to persist and remain active in the body, thus setting the stage for a new generation of CARs (i.e. second-generation CARs) [16]. Starting from this new discovery, a research team from the Memorial Sloan

Kettering (MSK) Cancer Center built the first effective CAR T cells,
directed against a prostate cancer antigen, demonstrating that these second-generation CARs T cells were able to survive, proliferate and kill prostate cancer cells in vitro and establishing the feasibility of CAR T cell therapy [17]. It was in 2003 the first description of human CAR T cells directed against CD19, which demonstrated the ability to kill leukemia cells in a mouse model. This was the first time that CD19 was shown as a feasible target for CAR T cells [18]. breast and lung cancer, were published [21]. The production of a new generation of CARs, so-called "armored CARs" (i.e. autologous T cells genetically engineered to secrete cytokine such as IL-12 upon antigen recognition), opened the way to the first phase I study of immunotherapy with armored CARs directed against the MUC16ecto antigen to treat recurrent high-grade serous ovarian, fallopian tube or primary peritoneal cancer (NCT02498912I), which is actually recruiting.
In 2017, the first CARs crossed the regulatory finish line, as the FDA approved CD19-directed T cells for the treatment of relapsed, refractory ALL in children and young adults.
In 2018, Dr. Sadelain and colleagues demonstrated that new engineering modalities, based on targeted nucleases like CRISPR, can be used to place a CAR at a specific genetic location in T cells, improving the manufacturing process and further enhancing the efficacy and safety of CAR T cells [22]. Nowadays, CAR T cell therapy represent one of the most popularly cited scientific advance, since it offers a further hope to our patients. However, we should attempt to remind that this leading discovery has been made possible by multiple little advances started more than 50 years ago. Like it has been said by the famed Isaac Newton "If I saw further than other men, it was because I stood on the shoulders of giants", our advances are made by thousands of little pieces of knowledge added by the perseverance and love of science of several eminent scientists.